| 绒山羊和绵羊源东毕吸虫ITS rDNA的PCR扩增及序列分析 |
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| 引用本文： | 艾琳,;王春仁,;唐剑栋,;林瑞庆,;朱兴全. 绒山羊和绵羊源东毕吸虫ITS rDNA的PCR扩增及序列分析[J]. 广东寄生虫学会年报, 2007, 0(3): 209-211 |
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| 作者姓名： | 艾琳, 王春仁, 唐剑栋, 林瑞庆, 朱兴全 |
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| 作者单位： | [1]华南农业大学兽医学院,广州510640; [2]黑龙江八一农垦大学动物科技学院,大庆163319 |
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| 基金项目： | 国家杰出青年科学基金（No.30225033）. |
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| 摘 要： | 目的扩增绒山羊和绵羊源东毕吸虫的ITS rDNA序列并进行分析比较。方法运用PCR方法，以保守引物BD1和BD2扩增从黑龙江绒山羊和绵羊体内分离的东毕吸虫（Oriemobilharzia spp．）rDNA的内转录间隔区（ITS-1及ITS-2）。PCR产物经测序后进行序列分析。结果黑龙江绒山羊和绵羊东毕吸虫的ITS序列总长均为875bp，其中ITS-1序列长为384Up，5．8S序列长为159Up，ITS-2序列长为332Up。黑龙江绒山羊和绵羊东毕吸虫的ITS序列相似性为99．9％，只有一个碱基差异，存在于ITS-2序列中。结论本研究在国际上首次报道了绒山羊和绵羊东毕吸虫的ITS序列，证实绒山羊和绵羊源东毕吸虫代表同一个种。这些结果为东毕吸虫分子生物学的进一步研究奠定了基础，并为寄生虫分子系统学研究提供了新资料。
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| 关 键 词： | 东毕吸虫 ITS序列 PCR 序列分析 |
PCR Amplification and Sequence Analysis of the ITS rDNA of Orientobilharzia spp. from Goat and Sheep in Hei Longjinang Province |
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| Affiliation: | AI Lin, WANG Chun-ren, TANG Jian-dong, LIN Rui-qing, ZHU Xing-quan (College of Veterinary Medicine, South China Agricultural University, Guangzhou 510640, China) |
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| Abstract: | Objective The aim of the present study was to obtain the ITS sequences of Orientobilharzia spp. from goat and sheep in Hei Longjiang province. Methods The DNA region spanning the first internal transcribed spacer （ITS-1）, 5.8S and the second internal transcribed spacer （ITS-2） of ribosomal DNA was amplified by PCR using a pair of conserved primers from individual samples from goat and sheep in Hei Longjiang province in China. The amplicons were sequenced directly and compared with the relevant sequences. Results The length of ITS-1, 5.8S and ITS-2 representing the two Orientobilhazia samples was 384 bp, 159 bp, and 332 bp, respectively. There was only a single base difference in the ITS-2 between the sample from goat and sheep. Conclusion Results from the present study demonstrate that Orientobiharzia from sheep and goat represent the same species. |
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| Keywords: | Orientobilharzia ITS sequence PCR sequence analysis |
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