| 热应激对A549细胞HSP70、XPA修复酶表达的影响 |
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| 引用本文： | 隆宗,;张林,;邬堂春. 热应激对A549细胞HSP70、XPA修复酶表达的影响[J]. 广东寄生虫学会年报, 2007, 0(6): 536-538 |
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| 作者姓名： | 隆宗, 张林, 邬堂春 |
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| 作者单位： | [1]广州市疾病预防控制中心,广州510080; [2]华中科技大学同济医学院,武汉430030 |
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| 基金项目： | 国家自然科学基金（No.30371204）. |
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| 摘 要： | 目的研究热应激作用下A549细胞热休克蛋白70（HSP70）和XPA修复酶的表达规律及其可能的意义。方法利用免疫印迹方法（Western blot）检测A549细胞中HSP70、XPA修复酶的表达水平。结果A549细胞接受不同温度（39、41、42、43℃）处理2h后,HSP70表达水平随温度的增高而逐渐增加,并在42℃应激时表达最高,与对照组相比有统计学意义（P〈0.05）;细胞接受42℃应激1～4h,HSP70表达水平随应激时间的增加而递增,与对照组相比,细胞在42℃应激2～4h时HSP70表达显著上调（P〈0.05）。两处理组细胞XPA修复酶表达水平均无明显变化（P〉0.05）。结论高温能诱导HSP70的表达,并呈一定的温度-效应和时间-效应关系,而对XPA修复酶未见明显影响。
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| 关 键 词： | 热应激 热休克蛋白70类 XPA修复酶 |
Effects of Heat Stress on HSP70 and XPA Repair Enzyme in A549 Cell Line |
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| Affiliation: | LONG Zong-yan, ZHANG Lin, WU Tang-chun (1. Guangzhou Center for Disease Control and Prevention, Guangzhou 510080; 2. Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China) |
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| Abstract: | Objective To examine the effect of heat stress on the expression of HSP70 and XPA repair enzyme in A549 cell line. Method Western-blot was used to detect the expression of HSP70 and XPA repair enzyme. Result At the temperature of 39-43℃, the levels of HSP70 were significantly increased and reached a peak value at 42℃. At 42℃, a time dependent （1-4 h） increase in the expression of HSP70 was observed. The expression of XPA repair enzyme was not changed after the heat stress treatment. Conclusion Heat stress exerts a temperature and time-dependent effect on the expression of HSP70. |
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| Keywords: | heat stress heat shock protein 70 XPA repair enzyme |
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