紫杉醇磁性脂质体纳米粒的制备

目的研究一种制备高载药量的紫杉醇磁性脂质体纳米粒的最佳条件，并对其质量进行检测。方法 通过共沉淀法制备Fe₃O₄纳米粒，同时施加超声处理减少粒子的软团聚合，增加粒子的分散度，对粒子表面进行改性，增加与脂质的结合，最后通过微乳液-低温固化法合成紫杉醇磁性脂质体纳米粒，并通过反相高压液相色谱法检测药物的载药量和包封率。结果紫杉醇磁性脂质体纳米粒为球形或近似球形，其悬浮液样品和冻干样品的粒径约在150～170 nm，药物包封率为98.29%。结论本法制备的粒子具有高质量磁化率、良好磁响应性，符合作为纳米磁靶向给药系统的条件。

Preparation of magnetic solid liposome nanoparticles of paclitaxel

Aim To study a new way to prepare high-dosage paclitaxel entrapped magnetic targeted nanoparticles and evaluate its quality. Methods Fe3O4 nanoparticles are prepared by co-depositing, at the same time ultrasonic is used to decrease soft agglomerate of nanoparticles and increase disperse level of it. The property of nanoparticles surface is improved to make the integrating of liposome and nanoparticle to be tighter, At last, paclitaxel entrapped magnetic solid liposome nanoparticles have been prepared by microemulsion-curing under low-temperature. The loading efficiency and encapsulating rate were determined by reverse-phase high-perfomance chromatography. Results The nanoparticles have spherical shape. Diameter of nanoparticle ranged from 150 nm to 170 nm. 98.29% of the drug is entrapped in the particle. Conclusion Magnetic susceptibility of nanoparticles is high, and the nanoparticles meet with the demand of targeted delivery system.