粉防己碱、清胰汤对重症急性胰腺炎猪血胃泌素、胃动素的影响

目的　应用去氧胆酸钠诱发香猪重症急性胰腺炎 (severe acute pancreatitis,SAP)模型 ,观察粉防己碱、清胰汤对 SAP香猪血胃肠激素水平的影响。　方法　用 32只贵州香猪 ,雌雄不拘 ,随机分为 4组 :生理盐水 (NS)对照组 ;粉防己碱 (tetrandrine,Tet)组 ;中药清胰汤 (chinese medicine qing yitang,CMQ)组 ;Tet+ CMQ联合组。各组受试动物术前禁食 12 h,禁水 6 h,无菌手术行门静脉、腔静脉插管 ,经胰管逆行加压推注 5 %去氧胆酸钠 (0 .5 ml/ kg体重 )复制 SAP模型 ,制模后 1h静脉滴注 NS或相应药物、胃管推注 NS或相应药物。分别于制模前 ,制模后 1h、6 h、12 h、2 4 h、4 8h、72 h、96 h同时采门静脉血和中心静脉血 ,分离血浆或血清 ,用放免法检测血浆胃动素和血清胃泌素水平 ;记录观察时间内 (96 h)各组动物的死亡率 ;取第 96小时动物的胰腺、十二指肠 ,作常规病理检查。　结果　与 NS组相比 ,粉防己碱、清胰汤均可降低 SAP香猪 96 h内的死亡率 ;可显著降低各时间点门静脉、中心静脉(除制模前、制模后 1h外 )血清胃泌素的浓度 (P<0 .0 1) ,可增加血浆胃动素的浓度 (P<0 .0 1) ;作用效果二者相当 (P>0 .0 5 ) ,而联合组的作用更为显著 (P<0 .0 1或 P<0 .0 5 )。用药组的胰腺、十二指肠病理变化也较 NS组明

Effects of tetrandrine,Qingyi Tang on blood levels of gastrin and motilin in pigs with severe acute pancreatitis

Objective To investigate the effects of tetrandrine,Chinese medicine Qingyi Tang(CMQ) on gastrointestinal hormones in miniature pigs with severe acute pancreatitis(SAP) induced by sodium deoxycholate. Methods 32 Guizhou miniature pigs were divided randomly into four groups ( n =8) ,including control group,tetrandrine group,CMQ group,and tetrandrine associating CMQ group. All experimental animals were fasted overnight without access to water for six hours before operation,and then portal vein and vena cava paracentesis was performed with sterile technique. The SAP model was induced by injecting 5% sodium deoxycholate (0.5 ml/kg weight) into the pancreatic duct at a constant pressure. One hour after the operation,saline,CMQ,tetrandrine or CMQ associating tetrandrine was administered accordingly by intravenous drip or by gastric catheterization. At pre-model,and 1 h,6 h,12 h,24 h,48 h,72 h and 96 h after inducement of SAP,blood was drawn from all pigs via the portal vein and vena cava at the same time. Serum levels of gastrin andplasma levels of motilin were tested by radioimmunoassay. The mortality of the pigs in different groups was recorded. Results Compared with NS group,tetrandrine and CMQ reduced the mortality significantly ( P < 0.05 or P < 0.01),and attenuated the increase of gastrin level and the decrease of motilin level both in the portal vein and vena cava. The effect of tetrandrine was similar to that of CMQ ( P > 0.05). The effect of their association was more significant than that of the other groups ( P < 0.05 or P < 0.01). Also,the pathologic morphology in the pancreas and duodenum improved in tetrandrine and CMQ groups. Conclusions The results of this study showed that tetrandrine and CMQ offered good therapeutic effects on pig SAP induced by sodium deoxycholate. The mechanisms might be related to their multi functions on the stressed body by decreasing the higher gastrin level to prevent the stress ulcer,and by increasing the lower motilin level,thus accelerating intestinal peristalsis and drainage of poisonous substances to prevent ileus and endotoxemia in SAP.