HPLC法同时测定温肾养肝颗粒中4种成分

目的建立HPLC法同时测定温肾养肝颗粒(肉苁蓉、乌药、白芍等)中4种成分的含有量。方法该药物50%甲醇提取液的分析采用AkzoNobel Kromasil C₁₈色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.2%磷酸为流动相,梯度洗脱;体积流量1 mL/min;柱温30℃;检测波长238、330 nm。结果去甲异波尔定、松果菊苷、芍药苷、毛蕊花糖苷分别在0.008 3～0.082 6 mg/mL (r=0.999 8)、0.021 4～0.213 8 mg/mL (r=0.999 8)、0.040 7～0.407 0 mg/mL (r=0.999 6)、0.005 9～0.058 7 mg/mL (r=0.999 6)范围内线性关系良好,平均加样回收率分别为99.55%、99.85%、99.93%、100.17%,RSD分别为1.43%、1.31%、0.97%、1.22%。结论该方法简便准确,重复性好,可用于温肾养肝颗粒的质量控制。

Simultaneous determination of four constituents in Wenshen Yanggan Granules by HPLC

AIM To establish an HPLC method for the simultaneous content determination of four constituents in Wenshen Yanggan Granules(Cistanches Herba,Linderae Radix,Paeoniae Radix Alba,etc.).METHODS The analysis of 50% methanol extract of this drug was performed on a 30 ℃ thermostatic AkzoNobel Kromasil C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 238 nm and 330 nm.RESULTS Norisoboldine,echinacoside,paeoniflorin and acteoside showed good linear relationships within the ranges of 0.008 3-0.082 6 mg/mL(r=0.999 8),0.021 4-0.213 8 mg/mL(r=0.999 8),0.040 7-0.407 0 mg/mL(r=0.999 6)and 0.005 9-0.058 7 mg/mL(r=0.999 6),whose average recoveries were 99.55%,99.85%,99.93% and 100.17% with the RSDs of 1.43%,1.31%,0.97% and 1.22%,respectively.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Wenshen Yanggan Granules.