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| HPLC法同时测定涤痰丸中7种成分 | |
| 引用本文： | 徐阳,单柏宇,徐伟男,鲍慧玮. HPLC法同时测定涤痰丸中7种成分[J]. 中成药, 2019, 0(1): 30-34 |
| 作者姓名： | 徐阳 单柏宇 徐伟男 鲍慧玮 |
| 作者单位： | 1.长春医学高等专科学校药品食品学院;2.白城市食品药品检验所;3.呼伦贝尔北方药业有限公司;4.长春中医药大学药学院 |
| 摘 要： | 目的建立HPLC法同时测定涤痰丸(牵牛子、大黄、黄芩)中没食子酸、儿茶素、咖啡酸、大黄酸、大黄素、阿魏酸、黄芩苷的含有量。方法该药物甲醇提取液的分析采用Agilent ZORBAX SB-C18色谱柱(250 mm×4. 6 mm,5μm);流动相甲醇-0. 1%磷酸,梯度洗脱;体积流量1. 0 mL/min;柱温30℃;检测波长254、278、320 nm。结果 7种成分在各自范围内线性关系良好(r> 0. 999 5),平均加样回收率98. 74%～100. 95%,RSD0. 86%～1. 84%。结论该方法简便准确,重复性好,可用于涤痰丸的质量控制。 |
| 关 键 词： | 涤痰丸 化学成分 HPLC |
Simultaneous determination of seven constituents in Ditan Pills by HPLC |
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| XU Yang,SHAN Bai-yu,XU Wei-nan,BAO Hui-wei. Simultaneous determination of seven constituents in Ditan Pills by HPLC[J]. Chinese Traditional Patent Medicine, 2019, 0(1): 30-34 | |
| Authors: | XU Yang SHAN Bai-yu XU Wei-nan BAO Hui-wei |
| Affiliation: | (College of Drug and Food,Changchun Medical College,Changchun 130031,China;Baicheng Municipal Institute for Food and Drug Control, Baicheng 137000,China;Hulunbuir Northern Pharmaceutical Co.,Ltd.,Hulunbuir 022150,China;College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China) |
| Abstract: | AIM To establish an HPLC method for the simultaneous content determination of gallic acid,catechin, caffeic acid,rhein,emodin,ferulic acid and baicalin in Ditan Pills ( Pharbitidis Semen,Rhei Radix et Rhizoma, Scutellariae Radix). METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent ZORBAX SB-C18 column (250 mm×4.6 mm,5μm),with the mobile phase comprising of methanol-0. 1% phosphoric acid flowing at 1. 0 mL /min in a gradient elution manner, and the detection wavelengths were set at 245,320,278 nm. RESULTS Seven constituents showed good linear relationships within their own ranges ( r > 0. 9995),whose average recoveries were 98. 74%- 100. 95% with the RSDs of 0. 86%-1. 84%. CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Ditan Pills. |
| Keywords: | Ditan Pills chemical constituents HPLC |
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