HPLC法同时测定麝香保心丸中间体中7种成分

目的建立HPLC法同时测定麝香保心丸(人工麝香、人参提取物、苏合香等)中间体中日蟾毒他灵、香豆素、肉桂酸、蟾毒他灵、蟾毒灵、华蟾酥毒基、酯蟾毒配基的含有量。方法该中间体甲醇提取液的分析采用Agilent Zorbax SB-C₁₈色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0～1.2 mL/min;柱温30℃;检测波长280 nm。结果 7种成分在各自范围内线性关系良好(r≥0.999 8),平均加样回收率98.24%～102.49%,RSD 0.93%～2.87%。结论该方法简便准确,重复性好,可用于麝香保心丸的质量控制。

Simultaneous determination of seven constituents in Shexiang Baoxin Pills intermediate by HPLC

AIM To establish an HPLC method for the simultaneous content determination of gamabufalin,coumarin,cinnamic acid,bufotaline,bufalin,cinobufagin and resibufogenin in Shexiang Baoxin Pills(Moschus Artifactus,Ginseng Radix et Rhizoma extract,Styrax,etc.)intermediate.METHODS The analysis of methanol extract of this intermediate was performed on a 30 ℃ thermostatic Agilent Zorbax SB-C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0-1.2 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r≥0.999 8),whose average recoveries were 98.24%-102.49% with the RSDs of 0.93%-2.87%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Shexiang Baoxin Pills.