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| 正交设计法优选骨肽注射液纯化参数的研究 | |
| 引用本文： | 刘永成,王一博,张丽英,杨杨,倪孟祥. 正交设计法优选骨肽注射液纯化参数的研究[J]. 药物分析杂志, 2011, 0(4) |
| 作者姓名： | 刘永成 王一博 张丽英 杨杨 倪孟祥 |
| 作者单位： | 中国药科大学生命科学与技术学院;黑龙江省食品药品检验检测所; |
| 摘 要： | 目的:探讨优选骨肽注射液提取的工艺条件。方法:采用正交设计法,骨肽注射液多肽含量及操作可行性为考察指标,对骨肽注射液提取过程中乙醇沉淀浓度、酸沉淀(pH 2.0～4.0)、碱沉淀(pH 6.8～7.2)3个因素进行筛选。结果:对于选定的三因素三水平,骨肽注射液最佳纯化参数组合为乙醇沉淀浓度70%、酸沉淀pH 4.0,碱沉淀pH 8.0,结果具有较显著的统计学意义。结论:根据骨肽注射液最佳提取条件对猪骨进行骨多肽提取,提取效率高,成本低。 |
| 关 键 词： | 骨肽注射液 生产工艺 正交设计 |
Study by orthogonal design method on optimizing purification process for ossotide injection |
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| LIU Yong-cheng,,WANG Yi-bo,ZHANG Li-ying,YANG Yang,NI Meng-xiang. Study by orthogonal design method on optimizing purification process for ossotide injection[J]. Chinese Journal of Pharmaceutical Analysis, 2011, 0(4) | |
| Authors: | LIU Yong-cheng WANG Yi-bo ZHANG Li-ying YANG Yang NI Meng-xiang |
| Affiliation: | LIU Yong-cheng1,2,WANG Yi-bo2,ZHANG Li-ying2,YANG Yang2,NI Meng-xiang1(1.School of Life Science & Technology,China Pharmaceutical University,Nanjing 210009,China,2.Heilongjiang Institute for Food and Drug Control,Harbin 150001,China) |
| Abstract: | Objective:To optimize the technological condition of extraction for ossotide injection.Methods:The content of polypeptides which was determined by UV and operational feasibility were regarded as the marker of detection.On the basis of initial research,the concentration of ethanol with ethanol precipitation,acid precipitation(pH 2.0-4.0) and alkaline precipitation(pH 6.8-7.2) were used to optimize the technological condition of extraction by orthogonal test.Results:The optimum extraction conditions were 70% ... |
| Keywords: | ossotide injection extraction process orthogonal design |
| 本文献已被 CNKI 等数据库收录！ | |