原发性肝癌全基因组杂合性缺失的研究

目的 研究肝癌全基因组等位基因杂合性缺失（ＬＯＨ）及其临床意义。方法 采用３８２对微卫星标志，对６５例肝癌２２条常染色体等位基因ＬＯＨ进行检测。结果 全组平均杂合子６８．１％。ＬＯＨ〉３０％的有１ｑ、３ｑ、４ｑ、８ｐ、１３ｑ、１６ｑ和１７ｐ。ＨＢｓＡｇ阳性者Ｄ４Ｓ２９６４ ＬＯＨ（６６％）较ＨＢｓＡｇ阴性者（３０％）高（Ｐ〈０．０５）；Ｄ３Ｓ３６８１和Ｄ１７Ｓ９３８ ＬＯＨ者术后复发率（９１％、８

Genome-wide loss of heterozygosity analyses in primary hepatocellular carcinoma

Objective To investigate genome wide loss of heterozygosity (LOH) and its clinical significance in primary hepatocellular carcinoma (HCC) in southern China. Methods LOH on 22 autosomes was investigated with 382 sets of microsatellite markers in 65 cases of HCC. Results The average rate of informative loci was 68.1%. More than 30% LOH was detected in loci on 17p (54.1%), 4q (48.1%), 16q (43.8%), 1q (38 3%), 8p (37.2%), 13q (33.7%), and 3p (30.8%). The frequency of LOH on D4S2964 (4q13 21) in HBsAg positive cases was significantly higher than that in HBsAg negative cases ( P <0.05). Cases with LOH on loci both D3S3681 (3p14 21) and D17S938 (17p13) had significantly higher rates of postoporative recurrence than those without LOH on these two loci (91% and 83% vs 52% and 65%, respectively). The 3 year survival rate was significantly lower in cases with LOH than in those without LOH on D17S938 ( P <0.05). Conclusions LOH status in HCC patients in southern China is similar to that reported in other countries and areas. However, we first identified the high frequency of LOH on chromosome 3p in HCC. Furthermore, the infection of hepatitis B virus (HBV) may be correlated with the loss of some tumor suppressor genes on D4S2964 (4q12 13), and some tumor suppressor genes may reside on loci D3S3681 (3p12) and D17S938 (17p13), relating with tumor recurrence and prognosis.