大鼠脑底动脉降钙素基因相关肽能神经纤维分布的形态学观察

目的　探讨 14只成年鼠和 14只老年鼠脑底主要动脉降钙素基因相关肽能神经纤维的分布特征。方法　应用免疫组织化学ABC法、神经交点计数法。结果　成年鼠脑底各主要动脉壁上均含有呈线状黑色的CGRP能免疫反应阳性神经纤维 ,分布密度以大脑前动脉最高 ,其次是大脑中动脉、大脑后动脉、基底动脉。各动脉间CGRP能神经纤维密度比较具有统计学意义 (P <0 .0 1) ,成年鼠脑底动脉壁CGRP能神经纤维以环行分布为主 ;老年鼠脑底动脉壁CGRP能神经纤维密度以大脑前动脉最高 ,走行方式以网状分布为主 ;老年鼠脑底各主要动脉壁CGRP能神经纤维分布密度低于成年鼠相应脑底动脉。结论　不同脑底动脉CGRP能神经纤维分布特征各异 ,老年鼠较成年鼠脑底动脉CGRP能神经纤维的分布密度为低

Morphometric Observation of Calcitonin Gene-related Peptide Innervation on Cerebral Arteries in Rats

Objective To study the innervation of calcitonin gene related peptidergic (CGRP ergic) nerve on cerebral arteries in adult and aged rats. Method Immunohistochemical ABC technique and counting number of nerve intersections were used. Results The immunopositive fibers with the black linear structure were observed on the anterior cerebral artery (ACA), middle cerebral artery (MCA), posterior cerebral artery (PCA), basilar artery (BA) in adult and aged rats, the density of immunoreactive fibers comes in the following order: ACA>MCA>PCA>BA. Statistical analysis indicates that there is significant difference in density between each two arteries The characteristic running patterns of the immunopositive fibers included circular, meshwork and longitudinal, i.e.mainly in circular in adult rats, in meshwork in aged rats,compared with the namesake artery of the adult rats CGRP grgic positive nerve fibers in ACA, MCA, PCA of aged rats decreased obviously, remained unchanged in BA. Students t test was used and P<0.01. There were different distribution characteristics on the different cerebral artery. Conclusion The density of CGRP ergic nerve fibers in aged rats is lower than that in adult rats,which suggests that CGRP ergic nerve played an important role in origination and development of aged cerebral vessel diseases.