| 双液相萃取法测定三七块根与茎叶中三七总皂苷含量 |
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| 引用本文： | 习峰敏,刘昌铭,李付名,浦周芳,刘赟心,赵文秀.双液相萃取法测定三七块根与茎叶中三七总皂苷含量[J].吉林医药学院学报,2010,31(1). |
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| 作者姓名： | 习峰敏 刘昌铭 李付名 浦周芳 刘赟心 赵文秀 |
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| 作者单位： | 吉林医药学院药学院,吉林,吉林,132013 |
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| 基金项目： | 吉林医药学院大学生科研基金(吉医学科字2008-08) |
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| 摘 要： | 目的 比较三七根和茎叶中三七总皂苷含量.方法 以三七总皂苷回收率为指标,设计正交试验,优选硫酸铵/聚乙二醇双液相体系的最优萃取条件,在最优条件下用香草醛/高氯酸比色法分别测定三七根和茎叶中三七总皂苷的含量.结果 硫酸铵/聚乙二醇双液相最优萃取条件为聚乙二醇分子量6 000,聚乙二醇浓度24%,硫酸铵浓度12%,pH值2.5,有较高的分配系数7.45,回收率82.4%.此条件下测得三七根和茎叶中三七总皂苷含量分别为11.24%和12.98%.结论 三七茎叶在某些方面可作为三七根的替代资源,可为合理开发利用三七资源提供依据.
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| 关 键 词： | 三七总皂苷 双液相体系 正交试验 含量测定 |
Determination of content of total saponins in Panpax Notoginseng roots and leaves by aqueous two phase system |
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| XI Feng-min,LIU Chang-ming,LI Fu-ming,PU Zhou-fang,LIU Yun-xin,ZHAO Wen-xiu.Determination of content of total saponins in Panpax Notoginseng roots and leaves by aqueous two phase system[J].Journal of Jilin Medical College,2010,31(1). |
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| Authors: | XI Feng-min LIU Chang-ming LI Fu-ming PU Zhou-fang LIU Yun-xin ZHAO Wen-xiu |
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| Institution: | School of Pharmacy;Jilin Medcial College;Jilin City;Jilin Province;132013;China |
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| Abstract: | Objective To compare the content of total saponins in Panpax Notoginseng roots and leaves.Methods Recovery rate was used as indicator to design orthogonal experiment to optimize the extraction conditions of total saponins in aqueous two-phase systems(ATPS) with polyethylene glycol(PEG) and ammonium sulfate.The content of total saponins in Panpax Notoginseng roots and leaves was detected by colorimetry with vanillin and perchlorate under the optimum extraction condition.Results The optimum extraction conditi... |
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| Keywords: | Panpax Notoginseng saponins aqueous two phase system orthogonal test determination of content |
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