迷迭香酸对大鼠中性粒细胞自由基生成和溶酶体酶释放的影响

目的:研究迷迭香酸对大鼠中性粒细胞自由基生成和溶酶体酶释放的影响。方法:利用紫外分光法、荧光分光法研究大鼠中性粒细胞自由基生成和溶酶体酶释放以及胞浆钙离子浓度。结果:迷迭香酸能抑制中性粒细胞超氧阴离子和过氧化氢以及脂质过氧化产物丙二醛(MDA)的产生,还能减少溶菌酶及β-葡萄糖醛酸苷酶的释放,而且,它对FMLP引起的中性粒细胞胞浆钙离子浓度的增加具有拮抗作用。结论:迷迭香酸抑制中性粒细胞呼吸爆发和脂质过氧化及通过降低细胞内钙离子浓度而抑制溶酶体酶的释放。

EFFECTS OF ROSMARINIC ACID ON FREE RADICAL PRODUCTION AND LYSOSOMAL ENZYME RELEASE FROM RAT PERITONEAL NEUTROPHILS

AIM: To study the effect of rosmarinic acid (RAD) on free radical production and lysosomal enzyme release of rat peritoneal neutrophils (RPN). METHODS: The production of oxygen free radicals and the release of lysosomal enzymes and the cytosolic [Ca²⁺]i level of RPN were determined with spectrophotometry and spectrophotofluorometry. RESULTS: RAD decreased superoxide anion production of RPN stimulated by formyl Met Leu Phe(FMLP) and phorbol 1,2 myristate 1,3 acetate (PMA), and inhibited hydrogen peroxide production of RPN stimulated by PMA. Also, RAD inhibited lipid peroxidation of RPN induced by Fe²⁺/cysteine and the release of lysozyme and β-glucuronidase after stimulation with FMLP. RAD decreased the cytosolic [Ca²⁺]i level of the neutrophils. CONCLUSION: RAD inhibits oxygen free radical production of the activated RPN. The inhibitory effect of RAD on the release of lysosomal enzymes might be mediated by inhibiting the increase of intracellular Ca²⁺in the neutrophils.