Triptolide-induced apoptosis by inactivating nuclear factor-kappa B apoptotic pathway in multiple myeloma <Emphasis Type="Italic">in vitro</Emphasis> |
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Authors: | Rong?Zeng Linlan?Zeng Yan?Chen Fei?Zhao Rui?Li Lu?Wen Email author" target="_blank">Chun?ZhangEmail author |
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Institution: | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,China |
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Abstract: | The effect of triptolide on proliferation and apoptosis of human multiple myeloma RPMI-8226 cells in vitro, as well as the roles of nuclear factor-kappa B (NF-κB) and IκBα was investigated. The effect of tritptolide on the growth
of RPMI-8226 cells was studied by MTT assay. Apoptosis was detected by Hoechest 33258 staining and Annexin V/PI double staining
assay. The expression of NF-κB and IκBα was observed by Western blot and confocal microscopy. The results showed that triptolide
inactivated NF-κB apoptotic pathway in human multiple myeloma RPMI-8226 cells. Triptolide at nM range induced proliferation
inhibition in a dose- and time-dependent manner and apoptosis in a dose-dependent fashion in RPMI-8226 cells. Besides, we
observed the inhibition of NF-κB /p65 in the nuclear fraction was correlated with the increase in the protein expression of
IκBα in the cytosol. These results suggested that triptolide might exhibit its strong anti-tumor effects via inactivation
of NF-κB/p65 and IκBα. |
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Keywords: | triptolide human multiple myeloma apoptosis NF-κB/p65 IκBα |
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