肥胖儿童血清脂蛋白(a)水平及载脂蛋白(a)的遗传表型研究

本文对肥胖儿血清脂蛋白水平及血清载脂蛋白多态性进行分析，以探讨肥胖儿与高脂蛋白血症的关系及其血清载脂蛋白遗传表型的分布特点。用酶联免疫吸附法５７名肥胖儿及其双亲血清脂蛋白含量，用十二守基碘酸钠－聚丙烯酰胺凝胶电泳和Ｗｅｓｔｅｒｎ ｂｌｏｔ免疫印迹技术测定载脂蛋白遗传表型。

Studies on Sera Lipoprotein(a) Levels and Genetic Phenotypes of Apolipoprotein(a) in Obese Children

This report presents the studies ofsera levels of lipoprotein(a) [Lp (a)] and genetic polymorphism of apolipoprotein(a)[Fapo(a)] in obese children. The aims of this study were to explore the relationship between obese children and high sera levelsof Lp(a) and the distributive characteristics of geneticphenotypes of apo(a).Enzyme-linked immunosorbent assay(ELISA) was used to measure sera levels of Lp(a)in 57 obese children and their parents. The genetic phenotypes of apo (a) were determined by SDS-polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot analysis, Of 75%subjects of obese children with high Lp(a) level, one side of their parents had high concentration of sera Lp(a).A total of 7 different phenotypes of apo(a) were determined from 41 obese children , including 5 different phenotypes of single-isoform and 2 different phenotypes of two-isoform,Obese children mostly had S1 and S2 phenotypes,but the controls generally had S4 phenotype.The similar reports have not been found in the recent5-year references. The study of genetichenotypes of apo(a) in obese children can be of great importance to protection and treatment of the cardiovascular and cerebrovascular disease induced by obesity.