| Effect of gamma irradiation on nuclear factor—kappa B in cultured bone marrow stromal cells |
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| 引用本文： | 朱波 罗成基 等. Effect of gamma irradiation on nuclear factor—kappa B in cultured bone marrow stromal cells[J]. 中国人民解放军军医大学学报, 2002, 17(1): 42-46 |
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| 作者姓名： | 朱波 罗成基 等 |
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| 作者单位： | [1]CancerCenterofPLA,XinqiaoHospital,ThirdMilitaryMedicalUniversity,Chongqing400037,China [2]InstituteofCombinedInjury,ThirdMilitaryMedicalUniversiy,Chongqing400038,China |
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| 基金项目： | Supported by the “Ninth Five” Obligatory Budget of PLA. No. 96L045 |
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| 摘 要： | Objective: To explore the effect of gamma irradiation on nuclear factor-kappa B in cultured bone marrow stromal cells. Methods: Immunocytochemistry, Western blot and electrophoretic mobility shift assay (EMSA) were used. Results: The expression of NF-kB in cultured mouse bone marrow stromal cells (BM-SCs) on the level of protein was elevated after exposure to 60Co in the dosage of 8. 0 Gy with the use of im-munocytochemistry and Western blot. The activity of nuclear factor-kappa B in cultured BMSCs was significantly increased after exposure to gamma irradiation by using EMSA. The activity peak was at the 4th h after irradiation. Conclusion: Our results suggest that the activation of nuclear factor-kappa B in the BMSCs after irradiation may be involved in the protection of BMSCs against apoptosis and in the recovery of hematopoiesis after radiation.
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| 关 键 词： | 辐射损伤 骨髓 血细胞生成微环境 NF－κB 骨髓基质细胞 |
Effect of gamma irradiation on nuclear factor-kappa B in cultured bone marrow stromal cells |
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| ZHU Bo,LUO Cheng-ji,Guo Chao-hua,CHENG Xiao-ming,ZOU Zhong-min,ZHOU Jin-ming. Effect of gamma irradiation on nuclear factor-kappa B in cultured bone marrow stromal cells[J]. Journal of Medical Colleges of PLA(China), 2002, 17(1): 42-46 |
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| Authors: | ZHU Bo LUO Cheng-ji Guo Chao-hua CHENG Xiao-ming ZOU Zhong-min ZHOU Jin-ming |
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| Abstract: | Objective: To explore the effect of gamma irradiation on nuclear factor-kappa B in cultured bone marrow stromal cells. Methods: Immunocytochemistry, Western blot and electrophoretic mobility shift assay (EMSA) were used. Results: The expression of NF-kB in cultured mouse bone marrow stromal cells (BM-SCs) on the level of protein was elevated after exposure to 60Co in the dosage of 8. 0 Gy with the use of im-munocytochemistry and Western blot. The activity of nuclear factor-kappa B in cultured BMSCs was significantly increased after exposure to gamma irradiation by using EMSA. The activity peak was at the 4th h after irradiation. Conclusion: Our results suggest that the activation of nuclear factor-kappa B in the BMSCs after irradiation may be involved in the protection of BMSCs against apoptosis and in the recovery of hematopoiesis after radiation. |
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| Keywords: | radiation bone marrow hematopoietic microenvironment NF-kB bone marrow stromal cells |
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