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| HPLC法测定天麻眩晕宁合剂中天麻素的含量 | |
| 引用本文： | 赵成. HPLC法测定天麻眩晕宁合剂中天麻素的含量[J]. 安徽医药, 2008, 12(1): 23-24 |
| 作者姓名： | 赵成 |
| 作者单位： | 安徽省六安市药品检验所,安徽,六安,237000 |
| 摘 要： | 目的建立HPLC法测定天麻眩晕宁合剂中天麻素的含量，以便有效控制其内在质量。方法采用高效液相色谱法VP-ODS柱（150mm×4．6mm，5μm）；流动相：乙腈-0．05％磷酸溶液（5：95），检测波长220nm；流速1．0ml·min^-1，柱温为室温。结果天麻素在3．42—170mg·L^-1（r=0．9998，n=6）范围内线性关系良好，平均回收率为98．85％，RSD=0．80％。结论该法简便，灵敏，重复性好，准确可靠，可用于天麻眩晕宁合剂中天麻素的含量测定。 |
| 关 键 词： | 天麻眩晕宁合剂 高效液相色谱法 天麻素 含量测定 |
| 收稿时间： | 2007-04-24 |
| 修稿时间： | 2007-04-24 |
Determination of gastrodin in tianma xuanyunning heji by HPLC |
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| ZHAO Cheng. Determination of gastrodin in tianma xuanyunning heji by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2008, 12(1): 23-24 | |
| Authors: | ZHAO Cheng |
| Affiliation: | ZHAO Cheng (Lu＇an Institute for Drug Control , Lu＇an 237000, China) |
| Abstract: | Aim To establish a method for the determination of gastrodin in tianma xuanyunning heji. Methods HPLC method was used. The samples were separated by VP-ODS column（ 150 mm×4.6 mm,5 μm）. The mobile phase is actonitrile-0.05% phosphoric acid solution（5：95） with the detection wavelength at 220 nm. Flow rate was 1.0 ml·min^-1, and the column temperature was indoor temperature. Results The linear range of gastrodin was 3.42 - 170 mg·L^-1 （ r = 0. 9998, n = 6 ）. The average recovery was 99.85 %, with RSD of 0.80%. Conclusion This method is simple, sensitive, accurate and can be used to determine the contents of gastrodin in tianma xuanyunning heji. |
| Keywords: | tianma xuanyunning heji HPLC gastrodin determination |
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