| 高效液相色谱法同时测定健康人尿液中草酸和枸橼酸的含量 |
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| 引用本文： | 彭婕a,葛卫红a,谈恒山a,孙西钊b.高效液相色谱法同时测定健康人尿液中草酸和枸橼酸的含量[J].中国现代应用药学,2007,24(1):72-74. |
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| 作者姓名： | 彭婕a 葛卫红a 谈恒山a 孙西钊b |
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| 作者单位： | 1. 南京大学医学院附属鼓楼医院临床药学室,南京,210008
2. 南京大学医学院附属鼓楼医院泌尿外科,南京,210008 |
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| 摘 要： | 目的建立HPLC同时测定人尿草酸和枸橼酸含量的方法。方法采用Agilent SB C18(250mm×4.6mm,5μm)色谱柱;Agilent zorbax extend-C18(4.6mm×12.5mm,5μm)为保护柱;以0.018mol.L-1磷酸二氢钾(含0.010mol.L-1四丁基硫酸氢铵和2.69×10-5mol/L EDTA,pH2.4~2.5)为流动相;检测波长210nm;流速1.5mL.m in-1;柱温25℃;进样量20μL;以50mg/mL磺基水杨酸沉淀尿蛋白。结果草酸最低检测限为0.4μg.mL-1。线性范围为1.563~100μg.mL-1,平均回收率为97.66%,日内及日间精密度RSD<13.50%。枸橼酸最低检测限为0.8μg.mL-1。线性范围为3.125~200μg.mL-1,平均回收率为97.71%,日内及日间精密度RSD<8.25%。结论该方法简便,灵敏度高,重复性好,可用于尿路草酸钙结石成因的研究。
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| 关 键 词： | 草酸 枸橼酸 高效液相色谱法 人尿液 |
| 文章编号： | 1007-7693（2007）01-0072-03 |
| 修稿时间： | 2005-06-24 |
Simultaneous Determination of the Contents of Oxalate and Citrate in Human Urine by RP-HPLC |
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| PENG Jie,GE Wei-hong,TAN Heng-shan,SUN Xi-zhaob.Simultaneous Determination of the Contents of Oxalate and Citrate in Human Urine by RP-HPLC[J].The Chinese Journal of Modern Applied Pharmacy,2007,24(1):72-74. |
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| Authors: | PENG Jie GE Wei-hong TAN Heng-shan SUN Xi-zhaob |
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| Institution: | a. Department of Clinical Pharmacy, b. Department of Urinary Surgery, the Affiliated Drum Tower Hospital of Nanfing University Medical School, Nanfing 210008, China |
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| Abstract: | OBJECTIVE To establish a method of simultaneously determining the contents of oxalate and citrate in human urine by reversed phase high performance liquid chromatography.METHODS To observe the nomal value of oxalate and citrate in human urine,they were de |
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| Keywords: | oxalate citrate HPLC human urine |
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