| 人hTERT基因的pCIneo真核表达载体的构建Q78 |
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| 引用本文： | 滕勇,李旭升,关玉成,李雁军.人hTERT基因的pCIneo真核表达载体的构建Q78[J].西北国防医学杂志,2011,32(5):335-337. |
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| 作者姓名： | 滕勇 李旭升 关玉成 李雁军 |
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| 作者单位： | 1. 兰州军区乌鲁木齐总医院全军骨科中心骨一科,新疆乌鲁木齐,830000
2. 解放军68202部队医院 |
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| 基金项目： | 兰州军区医药卫生科研计划资助项目(LXH2007014) |
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| 摘 要： | 构建人端粒酶逆转录酶（hTERT）片段的基因的pCIneo真核表达载体。方法：根据hTERTmRNA序列设计引物，人胚肾转化细胞系293总RNA反转录得到的cDNA为模板，PCR扩增后克隆到载体pCIneo上，并进行酶切鉴定及测序。结果-酶切结果表明hTERT基因已经插入pCIneo真核表达载体，测序结果显示，核苷酸序列的同源性为99．89％，氨基酸序列的同源性为100％。结论：成功构建hTERT基因的pCIneo真核表达载体，为其下一步的研究奠定了基础。
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| 关 键 词： | 人端粒酶逆转录酶 表达载体 基因转染 |
Construction of pCIneo eukaryotic expression plasmid of hTERT |
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| TENG Yong,LI Xu-sheng,GUAN Yu-cheng,et al..Construction of pCIneo eukaryotic expression plasmid of hTERT[J].Medical Journal of National Defending Forces in Northwest China,2011,32(5):335-337. |
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| Authors: | TENG Yong LI Xu-sheng GUAN Yu-cheng |
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| Institution: | TENG Yong,LI Xu-sheng,GUAN Yu-cheng,et al.(Department of Orthopedics,Urumqi General Hospital of Lanzhou Command,PLA,Urumqi 830000,China) |
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| Abstract: | Objective:To construct pCIneo eukaryotic expression plasmid of hTERT.Methods: The primers were designed according to hTERT mRNA sequence and the hTERT gene was amplified by RT-PCR using 293 cells as template.Then gene of hTERT was subcloned into vectors pCIneo and confirmed by DNA sequence analysis and restriction endonuclease digestion.Results: The analysis result of recombinant vector by restriction endonuclease digestion showed that hTERT gene has been correctly inserted into plasmid pCIneo.The homology ... |
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