| 复方降脂合剂的质控方法研究 |
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| 引用本文： | 张国华,;董伟林.复方降脂合剂的质控方法研究[J].中国城乡企业卫生,2014(5):98-100. |
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| 作者姓名： | 张国华 ;董伟林 |
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| 作者单位： | [1]天津市安定医院药剂科,天津300024; [2]天津医科大学,天津300024; |
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| 摘 要： | 目的通过测定合剂中芍药苷的含量从而建立复方降脂合剂的质量控制方法。方法采用HPLC法,十八烷基硅胶柱,以甲醇-水-冰醋酸（30：70：0.5）为流动相,流速1.0ml·min-1,柱温为室温,检测器：紫外检测器,检测波长230nm。结果芍药苷的线性范围为0.5-70μg·ml-1,y=41337x＋3301.2,相关系数r=0.9998,该方法精密度、重复性、回收率均符合要求。结论该HPLC法方法简便,结果准确可靠,重复性好,适用于复方降脂合剂中芍药苷的测定。
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| 关 键 词： | 芍药苷 HPLC法 含量测定 |
The First Exploration on Quality Control Methods of The Compound Lipidlowering Preparation |
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| Institution: | ZHANG Guo-hua, DONG Wei-lin( 1. Tianjin Mental Health Centre, Tianjin 300024, China;2. Tianjin Medical University) |
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| Abstract: | Objective To establish the quality control methods of compound lipid-lowering agents by the content determination of paeoniflorin. Methods In the study, HPLC （High Performance Liquid Chromatography） method was adopted,which requires column of C18 （ 150 mmx4.6 mm, 5txm） and the mobile phase that consist with methanol-H20-glacial acetic acid（30：70：0.5）. Flow rate was 1.0ml· min^-1, column temperature was 30℃, and the detective wavelength was set at 230 nm. Results There is a good linearity in the range of 0.5-701μ · ml^-1, y =41 337x ＋3 301.2, r =0.999 8, the method precision, reproducibility, average recovery are in line with the requirements. Conclusions The HPLC method is simple, accurate and reliable, reproducible. |
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| Keywords: | Paeoniflorin HPLC Determination |
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