黏着斑激酶对ERK介导的滋养层细胞侵袭行为的调节作用

目的:探讨黏着斑激酶(FAK)在细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)信号通路介导的滋养层细胞侵袭中的作用.方法:利用人绒毛外细胞滋养层(extravillous cytotrophoblasts,EVCT)细胞体外侵袭模型,加入不同浓度的黏着斑激酶抑制...

Regulation of invasion behavior of ERK-mediated trophoblast by focal adhesion kinase

ObjectiveTo investigate the role of focal adhesion kinase (FAK) in extracellular signal-regulated kinase (ERK) signaling pathway mediated invadsion of trophoblasts. MethodsWe established a human extravillous cytotrophoblasts in vitro invasion model. Different concentrations of herbimycin A（FAK inhibitor）and PD98059 (ERK inhibitor) were given to observe the influence on the growth of trophoblast cells, FAK, ERK phosphorylation, and trophoblast invasion abilities. ResultsThe expression of phosphorylated FAK in the extravillous cytotrophoblasts (EVCT) was inhibited by herbimycin A in a concentration-dependent manner and expression of phosphorylated ERK1/2 was also partially reduced. PD98059 had no effect on the expression of phosphorylated FAK. Herbimycin A and PD98059 suppressed the in vitro invasion of EVCT to various degrees. ConclusionERK signaling pathway may be the common pathway for many invasive signals,and play a key role in the regulation of trophoblast invasion.