紫花牡荆素对人结肠癌SW480细胞生长和凋亡的影响

目的观察紫花牡荆素（CAS）对人结肠癌SW480细胞生长和凋亡的影响。方法体外培养人结肠癌SW480细胞,平皿克隆形成法测定细胞锚定依赖性生长能力,PI染色流式细胞术（FCM）分析细胞凋亡率,Western Blotting分析CAS对上调死亡受体-5（DR5）、抑制核因子κB（NF-κB）（p65）蛋白表达的影响。结果平皿克隆形成法检测显示CAS呈浓度依赖性抑制SW480细胞生长。PI染色FCM结果表明CAS呈时间和浓度依赖性诱导SW480细胞凋亡。Western Blotting检测显示CAS呈时间和浓度依赖性上调死亡受体-5（DR5）表达和抑制核因子κB（NF-κB）活性。结论 CAS具有抑制人结肠癌SW480细胞增殖和诱导凋亡的作用,其诱发结肠癌细胞凋亡作用与DR5表达和NF-κB活性有关。

Effects of Casticin on the Growth and Apoptosis of Colon Carcinoma Cell Line SW480

Objective To observe the effect of casticin（CAS）on the growth and apoptosis of human colon cancer SW480 cells.Methods Human colon cancer SW480 cells were cultured in vitro.The capability of anchoring dependent growth in SW480 cells was determined using agar colony formation assay.Flow cytometry（FCM）analysis after PI staining and（FCM）was used to examine apoptosis rate in SW480 cells.Western Blotting was used to analyze the expression of DR5 and NF-κB（p65）protein.Results Agar colony formation assay showed that CAS inhibited growth of SW480 in a dose-dependent manner.The results of FCM analysis after PI staining showed that CAS induced apoptosis in SW480 cells in time-and concentration-dependent manner.Western Blotting analysis showed CAS up-regulated the expression of death receptor-5（DR5）and inhibition activity of nuclear factor kappaB（NF-κB）.Conclusion CAS can inhibit the proliferation and induce apoptosis of human colon cancer SW480 cells in vitro and this action was associated with up-regulation of the expretion of the death receptor-5（DR5）and inhibition of NF-κB activity.