肌注不同剂量环孢霉素A抑制大鼠角膜移植的免疫排斥反应

目的：探讨环孢霉素A不同剂量对大鼠角膜移植免疫排斥反应的影响。方法：以Wistar大鼠为供体，Lewis大鼠为受体建立角膜移植实验模型。将33只Lewis大鼠（33眼）随机分为A、B、C3组，每组11只。A组术后肌注CsA【1mg，（kg·d）】，B组给予CsA【10mg／（kg·d）】，C组给予等量不含药物的PBS，每次100ul，3次/d，连续用药10d。术后判断植片排斥情况，比较3组角膜植片的平均存活时间和新生血管评分。术后10d，病理组织学检查角膜的结构变化，并进行T细胞增殖实验，观察各组间T细胞增殖情况。结果：A、B组植片发生排斥反应的时间明显延迟，A组植片平均存活时间为（12．5±0．43）d，B组为（58±18．79）d，C组为（9±0．45）d，3组比较差异有统计学意义（P〈0．01）。B组术后各时间点角膜新生血管的生长明显低于C组（P〈0．05）。A组与c组除第6天外，其余时间点无统计学意义。A、B组角膜植片中炎性细胞和新生血管较c组减少。角膜A、B组T细胞增殖量明显低于C组（P〈0．05）。结论：10mg／（kg·d）CsA能够有效抑制大鼠角膜移植术后免疫排斥的发生。

Experimental studies of cyclosporine A in different doses on the prevention of penetrating ketatoplasty rejection in a rat model

Objective： To study the effects of cyclosporine A in different doses on corneal transplantation in a rat model. Methods： Lewis rats were used as recipients and Wistar rats were used as donors. Animals were randomly assigned to 3 groups： group A 1 mg / （kg.d）,IM], group B 10 mg/（kg.d）,IM] and group C （phosphate-buffered saline,IM）.100 ul CsA was given 3 times a day. The grafts was observed by slit-lamp microscope and corneal survival time and corneal neovascularization was recorded. 10 days after the operation, histochemical method was carried out on the procured specimens of cornea and the situation of T-cell proliferation was observed. Results. The mean survival time of group A, group B and group C was （12.5±0.43） d, （58±18.79）d and （9±0.45）d respectively. The rejection of group （A,B） were statistically postponed compared with that of control group （P〈0.01）. In group B,corneal neovaseularization was markedly reduced compared with group C （P〈0.05）.Except for the sixth day, corneal neovascularization were not signifieanly different in group A and group C. More inflammatory cell infiltration and new vessels were found in cornea in group C ten days after the surgery.The value of T cell proliferation in group A and B was significantly lower than group C （P 〈0.05）. Conclusion ：That in cyclosporine A can markedly prolong the corneal allografts survival in a rat model.