参附注射液对失血性休克大鼠肾脏血栓调节蛋白及内皮细胞蛋白C受体基因表达的影响

目的探讨参附注射液对失血性休克大鼠肾脏血栓调节蛋白（TM）及内皮细胞蛋白C受体（EPCR）基因表达的影响及可能的机制。方法成年健康SD大鼠50只，随机分为5组：正常对照组、实验对照组、失血性休克组、林格液复苏组、参附复苏组，每组10只。建立失血性休克大鼠模型。林格液组用3倍失血量的林格液复苏；参附组用含参附注射液（10ml/kg）和林格液组成的3倍失血量的液体复苏。休克后4h及复苏后3h处死大鼠，留取肾组织，用RT-PCR法检测TM及EPCR的基因表达。结果与正常对照组比较，实验对照组肾组织TM和EPCR的mRNA表达升高（P〈0.05），休克后明显升高（P〈0.01）；林格液组和参附组复苏后肾组织TM和EPCR的mRNA表达较休克组均下降，差异有统计学意义，林格液组下降更明显（P〈001）；林格液组与参附组比较差异有统计学意义（P〈0.05）。结论失血性休克时大鼠肾脏组织TM和EPCR表达增加，这与休克时存在内皮细胞功能损伤有关，参附注射液可以从基因水平影响大鼠肾组织TM和EPCR的mRNA表达，保护内皮细胞功能。

Study on Effects of Shenfu Injection on Thrombomodulin and Endothelial Protein C Receptor on Kindney in Hemorrhagic Shock Rats

Objective To investigate the effects of Shenfu injection on thrombomodulin (Tm) and endothelial protein C receptor (EPCR) on kindney and to clarify its possible mechanism in hemorrhagic shock rats.Methods In this study,fifty Sprague-Dawley (SD) rats were randomly divided into the following five groups:controls; sham shock; hemorrhagic shock (HS group); resuscitation with Ringer's solution (RS group); resuscitation with Shenfu injection (SF group) with ten rats per group. The rats in RS group and SF group we...