子痫前期患者血清及胎盘组织中人类白细胞抗原G的表达研究

目的:检测正常晚期妊娠妇女及子痫前期患者胎盘组织HLA-G的表达及血清中sHLA-G的浓度,探讨HLA-G在子痫前期发病中的临床意义。方法:用半定量逆转录-聚合酶链技术(RT-PCR)检测37例正常晚期妊娠妇女(正常妊娠组)及41例子痫前期患者(轻度20例,重度21例)胎盘组织中HLA-G mRNA的表达;并用ELISA检测血清sHLA-G浓度。结果:(1)子痫前期胎盘组织中HLA-G mRNA表达水平分别为:轻度0.402±0.104、重度0.329±0.09,明显低于正常妊娠组的0.628±0.117(P<0.01),轻、重度差异亦有统计学意义(P<0.05)。(2)子痫前期血清sHLA-G浓度:轻度45.5±11.9u/ml,重度31.2±10.3u/ml,均明显低于正常妊娠组的105.7±12.5u/ml(P<0.01),轻重度差异亦有统计学意义(P<0.01)。(3)子痫前期患者血清sHLA-G浓度与胎盘组织HLA-G mRNA表达水平呈正相关(r=0.702,P<0.01)。结论:子痫前期血清sHLA-G浓度及胎盘组织中HLA-G表达水平均明显降低,可能与子痫前期发病及病情轻重程度相关。

Studies on the human leukocyte antigen-G in maternal serum and placentas in pre-eclampsia

Objective： To investigate the expression of human leukocyte antigen-G （HLA-G） in maternal serum and placenta in normal and pre-eclamptic pregnants. Methods： The level of placenta HLA-G mRNA was measured by RT-PCR in 37 normal pregnants and 41 preeclamptic pregnants （20 mild,21 severe）. The serum sHLA-G concentration was measured by ELISA. Results： （ 1 ） The level of HLA-G mRNA of placenta in mild and severe group （0. 402 ±0. 104,0. 329 ±0.09）were significantly lower than that in control group（0. 628 ± 0. 117）（P 〈0.01）. There was also significant difference between mild and severe group （P 〈 0. 05）. （2） The maternal serum concentration of sHLA-G in mild and severe group （45.5 ± 11.9u/ml,31.2± 10.3u/ml） were significantly lower than that in control group （105.7± 12.5u/ml ）（P 〈0. 01 ）. There was also significant difference between mild and severe group （P 〈0.01 ）. （3） There was a positive correlation between the level of HLA-G mRNA and the concentration of sHLA-G in the same pregnant group （ r = 0.702, P 〈 0.01 ）. Conclusion ： The level of HLA-G mRNA in placenta and the concentration of sHLA-G in serum are all decreased in the pre-eclamptic pregnants, which may be related to the development of pre-eclampsia.