多发性骨髓瘤血清蛋白电泳与免疫学分型

目的对38例多发性骨髓瘤(MM)患者血清蛋白电泳、免疫电泳及免疫球蛋白进行定量分析,以探讨M蛋白、MM型别分布及其在MM中的临床诊断价值。方法血清蛋白和免疫电泳采用全自动琼脂糖凝胶电泳仪进行电泳和扫描;免疫球蛋白定量采用Beckman CS-免疫化学分析仪,以全自动方式进行速率散射比浊分析,测各样本的免疫球蛋白(IgG、IgA、Ig M)含量。结果38例MM全部检出M带,22例MM尿液检出本-周氏蛋白,检出阳性率为57.90%。血清免疫电泳分型IgGκ型14例,占36.84%;IgGλ型10例,占26.32%;IgAκ型5例,占13.16%;IgAλ型6例,占15.79%;Ig Mλ型3例,占7.89%,为最少。结论血清蛋白及免疫电泳能快速、准确地进行MM分型,对MM的诊断、病情判断、治疗及预后评估均具有重要的临床价值。

Immunology typing of serum protein electrophoresis on multiple myeloma

Objective To study the serum protein electrophoresis,immunofixation electrophoresis immunoglobulin to detect serum of 38 multiple myeloma(MM) patients to find out clinical diagnosis value of M protein and the type.Methods Serum protein electrophoresis,immunofixation eletrophoresis were detected by HYDRAGELPROTEIN,immunoglobulin by Beckman CS,to detect IgG,IgA and IgM.Results In 38 cases had M protein,22 cases had Bence-Jones proteins in urine, positive rate was 57.90%.Serum immunofixation eletrophoresis manifest:there were IgG kappa 14 case (36.84%),IgG Lambda 10 case (26.32%) ,IgA kappa 5 case (13.16%),IgA lambda 6 case (15.79%),IgM Lambda 3(7.89%).Conclusion Serum protein electrophoresis,immunofixation electrophoresis can type MM rapidly accurately and have great specificity,and play a crucal role in diagnosis and prognosis of MM.It may be used as a kind of technique to detect MM and to study clinical analysis.