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| 糖尿病大鼠海马生长抑素的研究 | |
| 引用本文： | Zhang XM,Zhu X,Yuan ZG,Yu J,Zhou JL. 糖尿病大鼠海马生长抑素的研究[J]. 中国医学科学院学报, 2003, 25(2): 204-206 |
| 作者姓名： | Zhang XM Zhu X Yuan ZG Yu J Zhou JL |
| 作者单位： | 1. 浙江大学医学院细胞生物学与组织工程学研究所,杭州,310006 2. The Medical College of Zhejiang University |
| 基金项目： | 浙江省卫生厅科研项目(2002B022)资助 |
| 摘 要： | 目的 观察糖尿病大鼠海马内神经元生长抑素基因表达产物mRNA改变,研究糖尿病对海马神经元内生长抑素表达的影响,探讨慢性糖尿病性脑病的发病机制。方法 SD大鼠通过腹腔注射链脲佐菌素建立糖尿病大鼠模型,运用原位杂交组织化学技术和计算机图像分析技术检测和比较糖尿病大鼠与正常大鼠海马神经元内生长抑素mRNA的表达。结果 糖尿病大鼠海马生长抑素mRNA阳性神经元细胞数量、阳性细胞的光密度及阳性细胞平均细胞面积均比正常大鼠显著减少。结论 糖尿病大鼠海马神经元的生长抑素表达减少,可能在糖尿病患者发生慢性痴呆等糖尿病慢性脑病中起一定作用。 |
| 关 键 词： | 生长抑素 海马 糖尿病 大鼠 |
| 修稿时间： | 2002-11-10 |
Somatostatin mRNA expression in hippocampus of diabetic rats model |
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| Zhang Xiao-ming,Zhu Xi,Yuan Zhang-gen,Yu Jun,Zhou Ji-lin. Somatostatin mRNA expression in hippocampus of diabetic rats model[J]. Acta Academiae Medicinae Sinicae, 2003, 25(2): 204-206 | |
| Authors: | Zhang Xiao-ming Zhu Xi Yuan Zhang-gen Yu Jun Zhou Ji-lin |
| Affiliation: | Institute of Cell Biology and Tissue Engineering, Medical College of Zhejiang University, Hangzhou 310006, China. zxm@zju.edu.cn |
| Abstract: | Objective To investigate the relationship between diabetes and somatostatin (SOM). Methods We established a streptozocin-induced diabetic rats model and studied the changes of SOM mRNA in hippocampi of diabetic and normal rats by using in situ hybridication and computer image analysis. We studied the mechanism of chronic diabetes encephalopathy by observing the changes of somatostatin mRNA in the hippocampus of diabetic rats was studied. Result The number, light density and average area of positive cells in dentate gyrus area of hippocampus in diabetes model were reduced significantly as compared with normal rats (P < 0.01). Conclusion A decline in SOM mRNA expression may play a role in chronic diabetes encephalopathy because of SOM effect in brain. |
| Keywords: | somatostatin hippocampus diabetes rat |
| 本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录！ | |