一氧化氮与腹膜透析腹膜血管增生的关系

目的探讨阿托伐他汀及螺内酯对腹膜透析时血管增生的影响及其机制。方法将40只雄性Wistar大鼠随机分成4组，每组10只。A组为对照组，每日腹腔内注入0．9％生理盐水20ml。B、C、D组于腹腔内每日注入4．25％百特透析液20ml，并于试验第7、14、21、28天分别加入乳酸盐红霉素6．25万U，同时，C组给予螺内酯100mg·kg-1·d-1灌胃，D组给予阿托伐他汀20mg·kg-1·d-1灌胃。30d后留取各组大鼠腹膜用免疫组织化学法检测腹膜血管增生情况，硝酸还原法检测腹膜透析液中一氧化氮（NO）浓度。结果与A组相比，B、C、D组腹膜血管增生显著加重，腹膜透析液NO浓度升高（P〈0．05）；与B组相比，C、D组腹膜血管增生显著减轻，腹膜透析液NO浓度降低（P〈0．05）。结论阿托伐他汀及螺内酯能有效的减少腹膜血管增生，可能与下调NO表达有关。

Relationship of nitric oxide and angiogenesis in peritoneal dialysis

Objective To investigate the effect of atorvastatin and spironolactone on angiogenesis and its mechanisms in peritoneal dialysis. Methods 40 male wistar rats were divided into 4 groups randomly, 10 in each group. Rats in group A were intraperitoneally infected with 0. 9% normal saline daily as control. Rats in group B,C and D were intraperitoneally injected with 4. 25% batter dialysate 20 ml daily,and infected with erythromycin lactobionate 62 500 units on the 7th, 14th, 21st and 28th days of the treatment. Additionally, rats in group C were given spironolactone 100 mg/kg daily by gastric gavage, those in group D were given atrovastatin 20 mg/kg daily by gastric gavage. Rats were sacrificed on the 30th day of the treatment. Angiogenesis expression by immunohistochemistry in peritoneal membrane were examined in these rats. Nitrate reductase assay the concentration of nitric oxide （NO） in peritoneal dialysis solution. Results Angiogenesis of peritoneal membrane was eminent in group B,C, D than in group A, so did the NO in the peritoneal fluid （P〈0. 05）. Compared with group B, peritoneal angiogenesis significantly reduced, NO concentration decreased in group C and D （P〈 0. 05）. Conclusion Atorvastatin and spironolactone can effectively reduce the peritoneal angiogenesis, may be associated with reduced expression of NO.