地甘口服液对免疫介导再生障碍性贫血小鼠胸腺细胞增殖 …

目的：研究中药复方地甘口服液对免疫介导再生生贫血小鼠模型胸腺淋巴细胞增殖周期的影响。方法：选用Ｂａｌｂ／ｃ小鼠为实验对象，在接受亚致死剂量射线照射后，立即注射ＤＢＡ／２小鼠胸腺、淋巴结混合细胞，形成免疫介导再障小鼠模型，分组处理后，摘取胸腺制成单细胞悬液，然后采用流氏细胞仪（ＦＡＣＳ）进行分析。结果：免疫介导再障小鼠胸腺细胞绝大部分停留在Ｇ０／Ｇ１期，Ｓ期和Ｇ２＋Ｍ期细胞显著减少，与正常对照组比较

Effect of Digan Oral Liquid on Thymic Lymphocyte Proliferation Cycle in Immune Mediated Aplastic Anemia in Mice

To study the effect of Digan oral liquid (DGOL) on cell proliferation cycle of thymic lymphocyte in immune mediated aplastic anemia (IMAA) in mice. Methods: Balb/c mice were used in the experiment, after having exposed under a sublethal amount of radiation, the mice were received injection of mixed cells of thymus and lymphnode of DBA/2 mice to form the IMAA models. The model mice were grouped and treated, then their thymus was taken to prepare the single cell suspension for analysis by flow cytometry. Results: Majority of thymocyte of IMAA mice stopped at the G 0/G 1 phase, and the cells in S and G 2 M phases were significantly less than those in the normal control group, P<0 01. Both DGOL and Siwu oral liquid could increase the differentiation and proliferation of thymocytes and reduce the apoptosis rate of them, but DGOL showed a better effect, P<0 05. Conclusion: DGOL could significantly promote the differentiation and proliferation of thymocytes and reduce the apoptosis rate of them in IMAA mice, the effect of its is superior to that of Siwu oral liquid.