MPO、NQO1基因多态性与急性白血病易感性的相关研究

本研究旨在探讨髓过氧化物酶（MPO）和醌氧化还原酶1（NQ01）基因多态性与中国甘肃人群急性白血病易感性的关系。用1：1配对病例一对照研究和连接酶检测反应（LDR）分型方法分析急性白血病病例组（150例）和对照组（150例无癌住院患者）MPO和NQ01的基因多态性，比较不同基因型与急性白血病易感性的关系。结果表明，病例组MPO-463A等位基因分布频率低于对照组，MPO（G-463A）各基因型在病例组与对照组中的分布差异显著（妒=11．828，P〈0．05，OR=0．368，95％CI=0．205-0．610）。病例组NQ01-609T等位基因分布频率高于对照组，NQ01（C-609T）各基因型在病例组与对照组中的分布差异显著（X^2=17．931，P〈0．05，OR=1．428，95％CI=1．237-3．339）。基因多态联合作用分析显示，MPO野生型兼具NQ01野生型者发生急性髓系白血病的风险降低至33．6％。结论：MPO、NQ01与急性白血病易感性相关，携带MPO（G-463A）突变基因型（GA／AA）可降低白血病的发病风险，携带NQ01（C-609T）突变基因型（TC／TY）可增加白血病的发病风险，MPO野生型与NQ01野生型者联合作用可进一步降低急性髓系白血病的发病风险。

Relationship of MPO and NQO1 Gene Polymorphisms with Susceptibility to Acute Leukemia

The aim of this study was to investigate the relationship of the gene polymorphisms of myeloperoxidase （MPO） and NAD （P）H：quinone oxidoreductase 1 （NQO1） with the susceptibility to acute leukemia（AL） in Chinese Gansu population. A 1：1 paired case-control study of 150 patients with acute leukemia and 150 cancer-free inpatients as a control was conducted to detect the polymorphisms of MPO and NQ01 by LDR techniques. The results showed that the MPO-463A genotype frequency in patient group was lower than that in control group, and there was significant difference of MPO （G-463A） genotype between patient group and control group （ X^2 = 11. 828, P 〈 0.05, OR = 0.368, 95 % CI = 0.205 -0.610）. The NQ01-609T genotype frequency in patient group was higher than that in control group, and there was significant difference of NQO1 （C-609T） genetype between patient group and control group （ X^2 = 17.931, P 〈 0.05, OR = 1. 428, 95% CI = 1. 237 - 3. 339）. The combined gene analysis showed that the AML risk in patients carry- ing the wild genotypes of MPO and NQ01 was dropped to 33.6%. It is concluded that the MPO and NQ01 gene poly- morphisms are associated with susceptibility to AL. The AL risk may decrease in patients carrying MPO （G-463A） mu- tant gene（ GA/AA）, while the AL risk may increase in patients carrying NQO1 （C-609T） mutant gene（TC/TT）. The combined effect of MPO and NQ01 wild genotypes may further decrease AL risk.