脂蛋白(a)循环免疫复合物诱导巨噬细胞胆固醇酯蓄积

目的探讨氧化、天然脂蛋白（a）Lp（a）]循环免疫复合物（IC）致动脉粥样硬化作用。方法采用人源的氧化、天然Lp（a）与异源的抗载脂蛋白B（apoB）结合制备IC，观察不同浓度的Lp（a）-IC对小鼠腹腔巨噬细胞胆固醇酯的蓄积和泡沫细胞形成作用。结果氧化、天然Lp（a）-IC均引起巨噬细胞内胆固醇酯大量堆积，其效果强于Ox-Lp（a）（P〈0．001），且随着剂量的增加而升高，并转化为泡沫细胞；而天然Lp（a）、抗apoB处理的巨噬细胞内未见胆固醇酯的堆积。结论Lp（a）-IC导致巨噬细胞转化为泡沫细胞，参与动脉粥样硬化形成。

Lipoprotein(a) immune complexes induce cholesteryl ester accumulation in human macrophages

Objective To investigate the atherogenic role of lipoprotein(a)Lp(a)]immune complexes (IC).Methods Lp(a)-IC were prepared by using human oxidized and native Lp(a) interaction with sheep antibodies against human apolipoproteinB, respectively.And the effect of Lp(a)-IC inducing cholesteryl ester accumulation in human macrophages was studied.Results Both oxidized and native Lp(a)-IC induced cholesteryl ester accumulation in human macrophages, the level of cellular cholesteryl ester induced by native and oxidized Lp(a)-IC were significantly higher than induced by oxidized Lp(a)(P<0.001). Furthermore, the macrophages were found translated into foams.The degree of cholesteryl ester accumulation in macrophages improved, along with Lp(a)-IC level increasing. Whereas native Lp(a) and apoB-IgG did not show any effect on the level of cellular cholesteryl ester.Conclusions Lp(a)-IC translate macrophages into foams and in atherosclerotic formation.