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| 性连锁性遗传病植入前诊断的临床前运用研究 | |
| 引用本文： | 钱卫平,李崎,田箐燕,宋丹,郑理光,林坚,邹红艳,张敏. 性连锁性遗传病植入前诊断的临床前运用研究[J]. 实用预防医学, 2005, 12(5): 1046-1048 |
| 作者姓名： | 钱卫平 李崎 田箐燕 宋丹 郑理光 林坚 邹红艳 张敏 |
| 作者单位： | 深圳罗湖医院不孕不育&遗传诊疗中心,广东,深圳,518001 |
| 摘 要： | 目的建立一个高效、稳定的性连锁性遗传病植入前诊断的方法。方法取成人外周血单个淋巴细胞和单卵裂球，采用多重巢式多聚合酶链技术同时扩增性别相关基因Amelogenin基因和IL-3基因。结果男性单个淋巴细胞有效扩增率为92．5％，女性单个淋巴细胞的有效扩增率为91％，准确率100％。单卵裂球的扩增效率为94．1％。结论巢式PCR具有较高的扩增效率和准确性，适于临床植入前诊断的运用推广。 |
| 关 键 词： | 多聚合酶链 单细胞 植入前诊断 |
| 文章编号： | 1006-3110（2005）05-1046-03 |
| 收稿时间： | 2005-06-11 |
| 修稿时间： | 2005-06-11 |
Preclinical Study of Preimplantation Genetic Diagnosis for Sex Linked Genetic Diseases |
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| QIAN Wei - ping, LI Qi, TIAN Jing-yan,et al.. Preclinical Study of Preimplantation Genetic Diagnosis for Sex Linked Genetic Diseases[J]. Practical Preventive Medicine, 2005, 12(5): 1046-1048 | |
| Authors: | QIAN Wei - ping LI Qi TIAN Jing-yan et al. |
| Affiliation: | QIAN Wei - ping, LI Qi, TIAN Jing-yan, et al. |
| Abstract: | Objective To develop a rapid, high efficient, reliable method for preimplantation genetic diagnosis of sex linked genetic diseases. Methods Multiplex nested PCR was used to amplify the sex linked amelogenin and IL - 3 genes from human single lymphocytes and single blastomere. Results The amplification efficiencies of multiplex nested POR of male and female single lymphocytes were 92.5 % and 91 % respectively. The PCR products of the lymphocyte were exactly the same as those of the donor gDNA. The amplification efficiencies of multiplex nested POR of single blastomere was 94.1%. Conclusion The multiplex nested PCR is conventional and sensitive in single cell and single blastomere, could be applied in preimplantation genetic diagnosis. |
| Keywords: | Polymerase chain reaction Single cell Preimplantation genetic diagnosis |
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