一种研究中西药抗炎作用的塑料环肉芽肿定量法

常用于造成实验性肉芽肿的方法有棉球法、滤纸片法及肉芽气囊法等。我们用Rudas 法加以改良,成功地造成肉芽肿,可用于中西药物的筛选和抗炎作用的研究。此法简单易行,介绍如下:材料与方法一、聚氯乙烯塑料环:即一般试剂瓶上用的塑料瓶塞,外径为15mm,内径14mm,边

A Modified Plastic Ring Method for the Quantitative Evaluation of Anti-inflammatory Effect of Drugs

The Rudas method was modified by using the plastic ring made of polyvinyl chloride.The outer and inner diameters of the ring were 15mm and 14mm respectively,with a height of 7mm.The plastic ring was em bedded in the hypodermis of the back of adult rats under ether anesthesia.After 7-10 days growth of granulation tissue was induced by the rings.Then the animals were killed and their granulation tissues were stripped The weights of the granulation tissues(M±SD)were 561.5±138.5mg and 526±138mg in the control groups.The growth of the granulation tissues was inhibited significantly by hydrocortisone,warm water extract and percolate of taraxacum mongolicum Hand-Mazt because of thief anti-inflammatory effect,M±SD was 106.1±150.9mg,360.8±69.9mg and 231.1±39.6mg respectively. The results of this experiment were not influenced by the weight and the sex of animals as well as the room temperature and the amount of food.This method was very simple,cheap,convenient and satisfactory for a semiquantitative evaluation of the anti-inflammatory effect of drugs.