艰难梭菌感染C57BL/6小鼠模型的建立方法研究

目的 建立C57BL/6小鼠抗菌药物诱导艰难梭菌感染模型，为更有效地认识人类艰难梭菌感染性疾病的发病机制及治疗措施提供依据。方法 C57BL/6小鼠经抗菌药物混合液预处理3天后，进行克林霉素腹腔注射。之后给予高、中、低浓度艰难梭菌ATCC43255悬液灌胃，建立艰难梭菌感染诱导肠损伤和结肠炎的CDI模型。观察小鼠的病死率，平均相对体重、结肠组织病理学评分和粪便艰难梭菌A&B毒素变化。结果 小鼠经抗菌药物处理和艰难梭菌灌胃后，出现腹泻，体重下降，粪便艰难AB毒素检测阳性。结肠病理切片可见炎性细胞浸润和组织坏死等结肠炎表现。随着灌胃菌液浓度的增加，小鼠症状越重，粪便毒素值越高。造模后存活下来的小鼠即使再次给予艰难梭菌灌胃也不再出现症状。结论 C57BL/6小鼠艰难梭菌感染模型与人类CDI病程变化相似，可进一步用于CDI防治措施的研究。

Established Clostridium Difficile Infected C57BL/6 Mouse Model

Objective To established a CDI C57BL/6 mouse model for realize the mechanisms and treatments of CDI. Methods C57BL/6 mice were challenged with C.difficile strain VPI 10463 after treatment with an antibiotic cocktail. Disease activity index, mean relative weight, pathological scores and toxins A&B in stool samples were measured. Results After Intragastric administration with antibiotics and C.difficile,mice had signs of CDI,such as diarrhea, weight loss, positive test for feces AB toxins.Colon pathological visible inflammatory cells infiltration and tissue necrosis colitis.Disease severity varied from fulminant to minimal in accordance with the challenge dose.Survived mice from an initial episode of CDAD showed no evidence of diarrhea or colitis after subsequent rechallenge with C.difficile. Conclusion C57BL/6 CDI mouse model is similar with human progression and can be further used to study the prevention and control of CDI.