一氧化氮在氯胺酮预处理对大鼠应激性溃疡保护中的作用

目的：观察氯胺酮预处理对大鼠急性胃黏膜病变（stress ulcer, SU）后一氧化氮（NO）的影响及胃黏膜溃疡指数（ulcer index，UI）的变化，探讨其可能的保护机制。方法：取健康雄性 Wistar 大鼠 40 只，随机分为5组，每组8只：正常对照组（N组），应激性溃疡组（S组），氯胺酮1、2、3组（K1、K2、K3组）。采用水浸束缚应激（WRS）方法制作 SU 模型，于应激 6h 后检测血浆、胃粘膜 NO 水平、UI、大体及光镜下胃粘膜损伤程度及组织学改变。结果：氯胺酮预处理组与 S 组比较胃粘膜损伤程度明显减轻，并且氯胺酮预处理组大鼠血浆、胃黏膜 NO 水平明显增加。结论：氯胺酮预处理对大鼠急性胃黏膜病变有保护作用，其机制可能与氯胺酮改变血浆、胃粘膜 NO 水平等因素有关

Effect of ketamine pretreatment on stress ulcer in rats

Objective To observe the effect of ketamine pretreatment on stress ulcer in rats and the changes of nitric oxide (NO) and ulcer index (UI), and explore the possible mechanism of protection. Methods Forty rats were randomly divided into five groups, normal group (group N), stress ulcer group (group S), and ketamine groups (group K1, K2, and K3). The murine model of water immersion restraint stress was constructed. Six hours after stress, plasma and gastric mucosal NO levels were detected, UI was measured, the degree of gastric mucosa injury was observed grossly, and the histological changes were examined microscopically. Results As compared with group S, the degree of gastric mucosa injury was markedly declined in ketamine groups while plasma and gastric mucosal NO levels were obviously increased. Conclusion Ketamine pretreatment has a protective effect on stress ulcer, whose mechanisms may involve the capacity of ketmine to alter the levels of plasma and gastric mucosal NO.