^3H—镰刀菌素C在大鼠体内的分布及与DNA的结合

镰刀菌素C(Fusarin C,FC)是Wiebe和Bjeldanes(1971)首先从玉米中分离得到的一种霉菌毒素,本实验室于1984年也从串珠镰刀菌的玉米培养基中分离出该毒素,FC对细菌和哺乳类动物细胞均有明显的致突变作用.为探讨FC的致癌作用及其与林县食管癌高发的关系,本实验初步研究~3H-镰刀菌素C(~3H-FC)在大鼠体内的分布、代谢及在体外与大鼠食管的DNA结合.

The metabolism and DNA binding of 3H-fusarin C in rats

In this paper, the distribution and elimination of 3H-Fusarin C (3H-FC) in rats and DNA-binding of FC in cultured explants of rat esophagus are reported. The radioactivity distribution in tissues was altered dynamically, and the highest levels of radioactivity were found in the intestine, stomach and liver after giving 3H-FC into the stomachs of rats. Kidney, bladder, esophagus and spleen followed. The radioactivity levels in the lung and brain were low. Radioactivity in the blood reached a peak three hours after giving 3H-FC to rats, but about 50% of the radioactivity remained in the blood even after 24 h. The total urinary excretion of radioactivity in rats was found to be about 30.7% within 48 h, and about 27.8% was present in the feces. Only 5.4% unchanged FC was excreted in the urine, while other metabolites of FC accounted for 94.6% of total urinary radioactivity. DNA-binding of FC occurred in rat esophageal explants.