| 雷公藤多苷调节肾组织p38MAPK信号通路改善糖尿病肾病肾小球炎症性损伤的作用和机制 |
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| 引用本文： | 黄燕如,万毅刚,孙伟,毛志敏,赵青,史喜苗,姚建.雷公藤多苷调节肾组织p38MAPK信号通路改善糖尿病肾病肾小球炎症性损伤的作用和机制[J].中国中药杂志,2014,39(21):4102-4109. |
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| 作者姓名： | 黄燕如 万毅刚 孙伟 毛志敏 赵青 史喜苗 姚建 |
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| 作者单位： | 南京中医药大学 中西医结合鼓楼临床医学院, 江苏 南京 210008;南京中医药大学 肾病研究所, 江苏 南京 210029;南京中医药大学 肾病研究所, 江苏 南京 210029;南京大学 医学院 附属鼓楼医院 中医科, 江苏 南京 210008;南京中医药大学 肾病研究所, 江苏 南京 210029;南京中医药大学 中西医结合鼓楼临床医学院, 江苏 南京 210008;南京中医药大学 肾病研究所, 江苏 南京 210029;南京中医药大学 肾病研究所, 江苏 南京 210029;南京中医药大学 中西医结合鼓楼临床医学院, 江苏 南京 210008;南京中医药大学 肾病研究所, 江苏 南京 210029;Department of Molecular Signaling, University of Yamanashi, Japan Yamanashi 409-3898 |
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| 基金项目： | 国家自然科学基金面上项目(81374030);南京市卫生青年人才培养工程项目 |
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| 摘 要： | 目的:探讨雷公藤多苷(multi-glycoside of Tripterygium wilfordii,GTW)在体内改善糖尿病肾病(diabetic nephropathy,DN)模型鼠肾小球炎症性损伤的作用和机制。方法:采用单侧肾切除联合腹腔注射链脲佐菌素(streptozotocin,STZ)建立DN模型。将大鼠随机分为3组:假手术组、对照组、GTW组,各5只。大鼠在造模成功后分别经灌胃给予蒸馏水(2 m L)或GTW悬浊液(50 mg·kg-1·d-1),每日1次,连续8周。各组大鼠自给药开始计时,第8周末处死。采集血液、尿液样本和肾组织,观察各组大鼠尿白蛋白、肾功能、肾小球形态特征、巨噬细胞(ED1+细胞)浸润以及肾组织肿瘤坏死因子(tumor necrosis factor,TNF)-α,白介素(interleukin,IL)-1β,p38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK),磷酸化p38MAPK(phosphorylated p38,p-p38MAPK),转化生长因子(transforming growth factor,TGF)-β1的蛋白表达水平。结果:GTW能改善DN模型鼠一般情况、体重,减少尿白蛋白,减轻肾小球硬化,抑制肾小球ED1+细胞浸润,下调肾组织TNF-α,IL-1β,p-p38MAPK,TGF-β1蛋白表达水平。结论:GTW在体内具有抑制炎症细胞浸润和炎症因子表达,减轻肾组织炎症性损伤的作用;GTW通过下调肾组织p38MAPK信号通路中关键信号分子——p-p38MAPK蛋白表达水平,抑制炎症信号通路活性,减少TGF-β1表达,从而,改善肾组织炎症性损伤。
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| 关 键 词： | 糖尿病肾病 雷公藤多苷 炎症 p38丝裂原活化蛋白激酶信号通路 |
| 收稿时间： | 2014/7/23 0:00:00 |
Effects and mechanisms of multi-glycoside of Tripterygium wilfordii improving glomerular inflammatory injury by regulating p38MAPK signaling activation in diabetic nephropathy rats |
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| HUANG Yan-ru,WAN Yi-gang,SUN Wei,MAO Zhi-min,ZHAO Qing,SHI Xi-miao and YAO Jian.Effects and mechanisms of multi-glycoside of Tripterygium wilfordii improving glomerular inflammatory injury by regulating p38MAPK signaling activation in diabetic nephropathy rats[J].China Journal of Chinese Materia Medica,2014,39(21):4102-4109. |
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| Authors: | HUANG Yan-ru WAN Yi-gang SUN Wei MAO Zhi-min ZHAO Qing SHI Xi-miao and YAO Jian |
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| Institution: | Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Department of Traditional Chinese Medicine, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing 210008, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210008, China;Research Institute of Kidney Disease, Nanjing University of Chinese Medicine, Nanjing 210029, China;Department of Molecular Signaling, University of Yamanashi, Yamanashi 409-3898, Japan |
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| Abstract: | |
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| Keywords: | diabetic nephropathy multi-glycoside of Tripterygium wilfordii inflammation p38MAPK signaling pathway |
| 本文献已被 CNKI 等数据库收录！ |
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