东亚钳蝎毒及其成分抗癫痫肽的抗惊厥作用

本文报道了河北产东亚钳蝎毒及从粗毒中纯化的抗癫痫肽的抗惊厥作用,本实验用咖啡因、美解眠、士的宁引起惊厥,用生理盐水作空白对照,安定做阳性对照。生理盐水对照组,惊厥发生率均达100%;严重惊厥发作率分别为50%、70%、50%;死亡率分别为40%、30%、50%,各组惊厥平均总持续时间为115min、59.5min、72min。安定对抗咖啡因惊厥的作用较小(P>O.05);但使美解眠和士的宁惊厥各项指标显著下降(P<0.05)。抗癫痫肽对抗咖啡因惊厥的作用较强,四项指标均显著下降(P<0.01),使美解眠惊厥各项指标亦明显下降(P<0.01),但稍弱;对抗士的宁惊厥的作用强度与安定相似(P<0.05)。蝎毒的抗惊厥作用较抗癫痫肽弱,与空白对照组比较差异不显著。

ANTICONVULSIVE EFFECTS OF THE VENOM OF BUTHUS MARTENSII KARSCH AND ANTIEPILEPSY PEPTIDE

Reported in this paper are the anticonvulsive effects of the venom collected from scor-pions of Buthus martensii Karsch from Hebei province and AEP, isolated from the venom. In our experiment the convulsion on mice was induced by caffeine, megimide and strych-nine. The control group received normal saline and diazepan. Normal saline control group's seizure rate was 100%, caffeine, megimide and strychninegroup's severe convulsion rates were 50%, 70%, 50%, death rates were 40%, 30%, 50%.The mean duration of each group was respectively 115min, 59.5min, 72min. The group of diazepam was found ineffective convulsion caused by caffeine. Diazepancaused a sharp decrease of four convulsion norms set with megimide and strychnine. AEP'santiconvulsion effect was stronger. AEP's effect degree of anticonvulsion induced by strychnine was similar to diazepam(p0.05).