帕金森病模型大鼠中脑腹侧被盖区多巴胺能神经元改变的实验研究

目的：探讨帕金森病（Parkinson’s disease，PD）大鼠模型中脑腹侧被盖区（ventral tegmentalarea，VTA）多巴胺能神经元的改变。方法：应用6-羟基多巴胺（6-hydroxydopamine，6-OHDA）注射右侧黑质致密区（substantia nigra compacta，SNc）制作PD大鼠模型，进行阿朴吗啡（apomorphine，APO）诱发行为学观察、电镜、尼氏染色观察中脑VTA神经元的改变、酪氨酸羟化酶（tyrosine hydroxylase，TH）免疫组织化学ABC观察其DA能神经元的改变并进行图像分析。结果：APO诱发PD大鼠模型异常旋转行为，尼氏染色见PD大鼠中脑VTA有神经细胞肿胀、坏死等变化，VTA TH阳性神经元数量减少，形态学改变。结论：中脑VTA DA能神经参与PD模型大鼠的改变；APO能诱导6-OHDAPD模型大鼠的旋转行为，其强弱可能与TH^＋神经元数量直接相关。

Experimental study on dopaminergic neurons in mesencephalic ventral tegmental area of parkinson disease rats

Objective: To study the changes of dopaminergic neurons in mesencephalic ventral tegmental area(VTA) of Parkinson disease(PD) rats. Methods: 6-hydroxydopamine (6-OHDA) was injected into right substantia nigra compacta (SNc) of rats for making PD model. The praxiology changes induced by apomorphine were observed, neuronal alteration of left mesencephalic VTA were studied adopting electron microscope and Nissl's staining, and the changes of DA neurons were analyzed by tyrosine hydroxylase(TH) immunohistochemistry and image analysis. Results: The model rats showed abnormal rotational behavior, while neurons of mesencephalic VTA swelled and necrosed, and the amount of TH positive neuron decreased obviously. Conclusions: Mesencephalic VTA DA neurons take part in the pathologic changes of Parkinson disease(PD)rats. Abnormal rotations of model rat can be induced by APO injection, and the degree of rotation correlate with the amounts of TH positive neuron.