脾虚证大鼠肝组织端粒长度的变化及其与氧化应激关系的探讨

目的:探讨脾虚证大鼠肝组织的端粒长度变化及其与氧化应激反应的相关性,进一步揭示脾虚衰老的病理机制。方法:采用饮食不节和劳倦过度结合法建立脾气虚证模型,然后分别采用苦寒法和辛热法建立脾阳虚和脾阴虚证模型。分别用硫代巴比妥酸(TBA)法、比色法和Southern blotting杂交法检测大鼠肝组织的丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)活性和端粒(Telomere)长度。结果:与正常对照组相比,脾阴虚模型组肝组织MDA含量升高明显,P<0.05;脾阳虚模型组肝组织GSH-Px活性明显降低,P<0.01;脾阳虚和脾阴虚模型组端粒长度明显缩短,P<0.05。结论:脾虚状态下,在机体过氧化反应异常增强,自由基积累攻击DNA、损伤端粒而导致端粒长度缩短。

Study on the Change about of Telomere in Liver Tissue of Deficiency in Spleen Model Rats and the Relationship between this Change and Oxidative Stress

Objective:To study the change about length of telomere in liver tissue of deficiency in spleen model rats and the correlativity between this change and oxidative stress;To reveal the pathological mechanism of senescence which was caused by deficiency in spleen.Methods:Deficiency of spleen-Qi models were established by the combined methods of nocontronling dier and overfatigue.then,the methods of bitter cold and pungent warm were used to found deficiency of spleen-yin and deficiency of spleen-yang models.TBA,colorinetry and Southern blotting weredifferently used to detect the content of MDA,the acticity of GSH-Px and the length of telomere in liver tissue of rats.Results:Compared with normal control group,the content of MDA in liver tissue of deficiency in spleen-yin group increased obviously,P<0.05;The activity of GSH-Px in liver tissue of deficiency in spleen y-ang group decreased remarkably,P<0.01;The length of telomere of deficiency in spleen-yin model group and deficiency in spleen-yang model group shorteded apparently,P<0.05.Conclusion:In the state of deficiency in spleen,the reactivety of peroxide in organismreinforces abnormality,the accumulation of free radical attacks DNA and injurys telomere,which induces the length of telomere shortens.