白细胞介素-6基因启动子-174G/C、-572C/G多态性与冠心病的相关性

目的研究白细胞介素-6(IL-6)基因启动子-174G/C和-572C/G多态性在冠心病患者及正常人中的分布。方法应用聚合酶链反应-限制性片段长度多态性技术检测90例冠心病患者与95名健康成年体检者的IL-6基因启动子-174和-572的多态性,比较两组IL-6启动子基因型和等位基因的分布频率。结果IL-6基因启动子-174位点的GG、CG和CC基因型在冠心病患者中分别为100、0%、0;在健康成年者中分别为98.95%、1.05%、0。冠心病组与对照组基因的分布频率差异无统计学意义;-572位点的CC、CG和GG基因型在冠心病组中分别为54.44%、43.33%、2.22%;在健康成年者中分别为71.58%、27.37%、1.05%。两组的基因分布频率差异有统计学意义。结论IL-6基因启动子的-174位点变异率极低,其变异与冠心病的发病无明显相关性;而启动子-572位点的多态性分布与冠心病的发病具有一定程度的相关性。

Association of polymorphism in Interleukin-6(IL-6)gene promoter region with morbidity of coronary artery disease

Objective To detect the distribution of the site-174G/C and the site-572C/G in interleukin-6 gene promoter region in patients with coronary artery disease and normal controls.Methods Interleukin-6 gene promoter region polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism in patients with coronary artery disease(n=90)and normal controls(n=95).Results To the site-174,the frequencies of GG,GC and CC genotypes were 100%,0 and 0 in patients with coronary artery disease,while 98.95%,1.05% and 0 in normal controls.No significant difference was seen in the distribution frequency of interleukin-6 genotype between patients with coronary artery disease and normal controls.To the site-572 the frequencies of CC,CG and GG genotypes were 54.44%,43.33% and 2.22% in patients with coronary artery disease,while 71.58%,27.37% and 1.05% in normal controls.A significant difference was seen in two groups.Conclusion Mutation of the site-174 in interleukin-6 gene promoter region was rare.Its distribution frequency has nothing to do with morbilty of coronary artery disease.While to the site-572,its distribution frequency of genotype has something to do with morbilty of coronary artery disease.