骨髓多能干细胞移植治疗股骨头缺血性坏死的实验研究

目的 探讨骨髓多能干细胞移植治疗股骨头坏死的疗效和机制.方法 选用日本大耳白兔60只,随机分为A、B、C 3组,制成激素性股骨头坏死模型.A组兔抽取骨髓体外分离后,将骨髓干细胞注入钻孔减压的左侧股骨头内,右侧仅钻孔减压;B组模型末治疗组;C组为正常组.各组动物于治疗后8周处死,处死前后分别进行各种指标检测,包括钼靶X线摄影、CT扫描、MR扫描、常规HE染色切片、电镜扫描超微结构观察.结果 股骨头穿刺骨髓干细胞移植后8周,骨质密度X线、CT和MR明显好转.病理组织学表现空骨陷窝减少,成骨细胞增多,新骨形成.电镜下可见成骨细胞内细胞器丰富,空骨陷窝减少.经骨髓多能干细胞移植治疗后显示股骨头修复效果更佳.结论 骨髓多能干细胞移植治疗股骨头坏死能加速兔股骨头的修复进程,对股骨头坏死具有较高的治疗价值.

An experimental study on the treatment of femoral head necrosis with transplantation of marrow stem cells

Objective To investigate the effect and mechanism of transplantation of marrow multifunction stem cells in treating femoral head necrosis. Methods Sixty japanese rabbits were divided into A,B and C groups randomly. After creation of the models of hormone induced necrosis of femoral head; A group was designated as the treatment, B as the control and C as the normal groups. The bone marrow of A group was extracted and isolated and then injected into the left femoral head and the right femoral head was decompressed by drilling only. The rabbits were killed at 8 weeks after the treatment and changes in various parameters were observed, including imaging data of molybdenum target films, CT and MRI; routine pathology with HE staining and uhrastructural alteration by election microscopy. Results Eight weeks after the treatment of transplantation of marrow multifunction stem cells, the X-ray showed only a little change but the typical appearances were revealed by CT and MRI. Pathohistolagic manifestation demonstrated decrease of empty bone lacuna , increase of osteoblast and new bone formation. Election microscopy displayed abundant organelles in osteoblasts with few empty bone lacuna, in addition the tansplantation of marrow muhifunction stem cells could obtain better reconstraction for the involved femoral head. Conclusions The treatment of transplantation of marrow muhifunction stem cells in femoral head necrosis could accelerate the process of repairing, worthy to be acknowledged as a good and valuable management in rabbits.