用PCR技术检测大鼠弓形虫DNA

目的 探讨弓形虫病大鼠外周血弓形虫DNA检测的意义。方法 自行设计一对引物，用聚合酶链反应技术扩增弓形虫P30基因的一段保守序列。结果 设计的这对引物对健康人、大鼠、小鼠外周血白细胞以及阴道毛滴虫、溶组织内阿米巴均不能扩增，表明具有特异性。反应体系经35个循环扩增，可检测到2条弓形虫DNA，表明具有较高的敏感性。结论 PCR法对大鼠弓形虫感染可做出早期诊断。

Detection of DNA of Toxoplasma gondii in Rat by Using Polymerase Chain Reaction

Objective To study the significance of DNA of Toxoplasma gondii in peripheral blood. Methods DNA of T.gondii in peripheral blood of 50 infected rats was detected by polymerase chain reaction. A pair of primers was designed, according to the sequence P30 gene specific to T.gondii, to amplify DNA from T.gondii by PCR. Results The primers amplified DNA specifically from T.gondii and could not amplify DNA from humans, uninfected rat and mouse and from Trichomonas vaginalis and Entamoeba histolytica. DNA of two Toxoplasma parasites was detected by 35 cycles of amplification, indicating a fair sensitivity of the PCR system. Conclusion PCR may have a value for early diagnosis of T.gondii infection in rat.