奥曲肽对人胃癌BGC-823细胞增殖和凋亡影响的研究

目的：研究生长抑素类似物奥曲肽（octreotide，OCT）对胃癌BGC-823细胞增殖、凋亡、细胞周期的影响及其可能的作用机制。方法：不同浓度的OCT作用于体外培养的人胃癌BGC-823细胞，以5～FU作为阳性对照，应用M1Tr法分析细胞增殖的抑制作用，流式细胞仪测定细胞周期分布和凋亡率，AO—EB染色观察细胞形态变化。结果：OCT对BGC-823细胞的生长、增殖产生抑制作用，该抑制作用具有量效、时效关系和饱和性；OCT作用后细胞呈典型的凋亡形态学改变，流式细胞仪检测的细胞凋亡率和AO—EB染色测得细胞凋亡率与对照组相比较差异有显著性（P〈0．05）；BGC-823细胞经OCT作用后，G1期细胞数明显增加，S细胞数明显减少，细胞被阻滞于G1／S期。结论：10^-5-10^-3g/L浓度的OCT能够抑制胃癌BGC-823细胞增殖，机制可能与其诱导肿瘤细胞的凋亡和出现G1／S期阻滞有关。

Effect of octreotide on cells proliferation and apoptosis in human gastric cancer cell line BGC - 823

Objective：To study the effect of octreotide（OCT） on proliferation,apoptosis and cell cycle in human gastric cancer cell line BGC - 823 in vitro, and further to explore the possible mechanism of inhibition. Methods： Human gastric cancer cell line BGC - 823 was treated with Octreotide. 5 - FU was used as positive control. MTT assay and AO - EB fluorescent staining as well as flow cytometry were performed in this study. Results： Octreotide inhibited the growth and proliferation of BGC - 823 in vitro within certain concentrations. The suppression was dose - dependent, time - dependent, and had staturability. Morphological variations of apoptosis were observed after treated with OCT, the apoptotic rate measured by AO -EB fluorescent staining and flow cytometry had statistical significance compared with negative group（ P 〈 005 ）. G1 phase rate gradually increased, S phase rate gradually decreased, cell cycle was arrested at Glphase in human gastric cancer cell line BGC -823 treated by OCT. Conclusion： Octreotide can inhibit the proliferation of human gastric cancer cell line BGC - 823 in vitro. It might be related with inducing apoptosis and arresting cell cycle at G1/S phase.