Influenza virus-induced alterations of cytochrome P-450 enzyme activities following exposure of mice to coal and diesel particulates |
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| Authors: | J Rabovsky D J Judy D J Rodak M Petersen |
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| Affiliation: | 1. Center for Biofluid and Biomimic Research, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea;2. Gandhi Medical College, Secunderabad, Telangana 500003, India;3. Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea;1. Unidad de Investigación, Desarrollo e Innovación en Genética y Biología Molecular, Universidad Simón Bolívar, Barranquilla, Colombia;2. Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil;3. Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro, Brazil;4. Research group in Environmental Management and Sustainability, Faculty of Environmental Sciences, Universidad de la Costa, Barranquilla, Colombia;5. Universidade do Sul de Santa Catarina, Pró-Reitoria de Ensino, de Pesquisa e de Extensão, Pedra Branca, 88137900 Palhoça, SC, Brazil;6. Laboratório de Implantação Iônica, Instituto de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil;7. Laboratório de Química Ambiental e Oleoquímica, Programa de Pós-Graduação em Química, Universidade Federal do Rio Grande dos Sul (UFRGS), Porto Alegre, RS, Brazil;8. Laboratório de Genética Toxicológica, Universidade Luterana do Brasil (ULBRA), Canoas, RS, Brazil;9. Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Caxias do Sul, RS, Brazil |
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| Abstract: | ![]()
We have investigated a relationship between two detoxication systems, metabolic detoxication through the cytochrome P-450 (P-450) pathway and resistance to infection through interferon (IFN), in mice infected with influenza virus following exposure to coal dust (CD) and diesel exhaust (DE) particulates. Mice were exposed by inhalation to filtered air (FA; control), CD, or DE for 1 month and then inoculated intranasally (IN) with influenza virus. During infection, 7-ethoxycoumarin deethylase (7ECdeEt'ase) and ethylmorphine demethylase (EMdeMe'ase) (monooxygenases), and NADPH cytochrome c reductase (NADPH c red'ase) were measured in liver microsomes. Temporal patterns of enzyme activities were observed with control animals. EMdeMe'ase and NADPH c red'ase exhibited peak values at Day 4 postinfection (27.6 and 482 nmole/min/mg protein, respectively), compared to initial activities (9.1 and 307 nmole/min/mg protein, respectively). 7ECdeEt'ase activity decreased between Days 1-3 postvirus infection and thereafter returned to the original value (1.7 nmole/min/mg protein). When the mice were first exposed to CD or DE particulates for 1 month prior to influenza infection, changes in enzyme temporal patterns were observed. The increased EMdeMe'ase activity at Day 4 was not observed in mice exposed to CD and was reduced in mice exposed to DE. Preexposure to either particulate resulted in the abolition of the increased Day 4 activity of NADPH c red'ase. The 7ECdeEt'ase postinfection temporal pattern was not affected by a preexposure to either particulate. Estimates of the enzyme activities after the 1-month exposure to FA, CD, or DE but before virus infection indicated no changes due to particulate exposure alone. Under these conditions of particulate exposure and virus infection, serum IFN levels in the mice used in this study peaked at Days 4-5 and were unaffected by the 1-month preexposure to CD or DE (Hahon et al., (1985). The data suggest the relationship that exists between metabolic detoxication and resistance to infection in normal mice was altered during a short-term preexposure to CD or DE. |
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