Endothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes |
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| Authors: | Ramla Gary Daniela Amelio Filippo Garofalo Gia Petriashvili Maria Penelope De Santo Yuen Kwong Ip Riccardo Barberi |
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| Affiliation: | 1.Physics Department, University of Calabria, Rende, 87036, Italy;2.Department of Biology Ecology and Earth Science (B.E.ST), University of Calabria, 87030 Arcavacata di Rende, CS, Italy;3.Institute of Cybernetics of the Georgian Technical University, Tbilisi, 0175, Georgia;4.CNR-Nanotec UOS di Cosenza, c/o University of Calabria, Rende 87036, Italy;5.Department of Biological Sciences, National University of Singapore, Kent Ridge, Singapore 117543, Republic of Singapore |
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| Abstract: | ![]()
Immunofluorescence is a biological technique that allows displaying the localization of the target molecule through a fluorescent microscope. We used a combination of gold nanoparticles and the fluorescein isothiocianate, FITC, as optical contrast agents for laser scanning confocal microscopy imaging to localize the endothelial-like nitric oxide synthase in skeletal muscle cells in a three-dimensional tissue phantom at the depth of 4µm. The FITC detected fluorescence intensity from gold-nanoparticles-labelled cells was brighter than the emission intensity from unlabelled cells.OCIS codes: (170.1790) Confocal microscopy, (280.1415) Biological sensing and sensors |
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