HPLC法测定通滞苏润江胶囊中番泻苷A、B的含量

目的：建立通滞苏润江胶囊中番泻苷A、B的含量测定方法.方法：采用HPLC法对通滞苏润江胶囊中的番泻苷A、B含量进行测定,SinoChrom ODS BP色谱柱（250 mm×4.6 mm,5μm）,流动相乙腈-5 mmol·L-1四庚基溴化铵的醋酸.醋酸钠缓冲液（pH5.0）（1→10）（37：63）,流速：1.0 ml·min-1,柱温：40℃,检测波长：340 nm.结果：番泻苷A进样量在41.971～2 098.552 ng的范围内线性关系良好（r=0.999 9）,平均回收率为99.52％（RSD=1.39％,n=6）;番泻苷B进样量在42.781～2 139.046ng的范围内线性关系良好（r=0.999 9）,平均回收率为99.36％（RSD=1.45％,n=6）.结论：方法简便快捷,适用于该制剂中番泻苷A、B的含量测定.

Determination of Sennoside A and B in Tongzhi Surunjiang Capsules by HPLC

Objective： To establish an HPLC method for the determination of sennoside A and B in Tongzhi Surunjiang capsules. Methods： The contents of sennoside A and B were determined by HPLC method. A SinoChrom ODS BP column was used. The mobile phase was acetonitrile-5 mmol · L-1 tetraheptylammonium bromide（37： 63）with the flow rate of 1.0 ml · min- 1 and the column tem- perature of 40℃. The detection wavelength was set at 340 nm. Results： The linear range of sennoside A was 41. 971-2 098. 552 ng（ r = 0.999 9）, and the average recovery was 99.52% with RSD of 1.39% （n = 6）;the linear range of sennoside B was 42.781- 2 139. 046 ng（ r = 0. 999 9）, and the average recovery was 99.36% with RSD of 1.45% （n = 6）. Conclusion： The method is simple and fast. It can be used as the determination method of sennoside A and B in Tongzhi Surunjiang capsules.