缺血预处理对家兔脑缺血保护效应的实验研究

目的 探讨缺血预处理脑保护效应，以及神经细胞凋亡与缺血性脑损害的关系。方法 家兔１５只，随机分为３组，对照组，缺血组，缺血预处理组。Ａ组只做手术操作，Ｂ组采用二血管夹闭全脑缺血１０分钟，Ｃ组在缺血前增加缺血预处理２分钟再灌注３０分钟。对比观察缺血后３天海马ＣＡ１区神经元密度和缺血细胞数，同时使用ＴＵＮＥＬ原位标记法，检测缺血３天后海马区的凋亡细胞。

Experimental study on the protective effects of ischemic preconditioning on global braim ischemia in rabbit

Objective To determine the protective effects of ischemic preconditioning (IPC) on the brain ischemic injury,and to investigate the relationship between apoptosis and brain ischemic injury Methods Fifteen rabbits were randomly divided into three groups group A (n=5);for operative control,group B (n=5) global ischemia for 10min by two vessels occlusion model (bilateral common carotid artery occlusion combined with systemic hypotension),and group C (n=5) ischemic preconditioning for 2 min and reperfusion for half an hour before ischemia Ischemic neurons and neuron density of the hippocampal CA1 region were examined and measured 3 days later Apoptoic cells were measured with paraffin sections stained by TUNEL method Results In group C,neuron density was markedly higher than that in group B (P<0 05) In group C,ischemic neuron number was markedly less than that in group B (P<0 01) IPC protective effects for the medial,middle and lateral subsections of the hippocampal CA1 region were 88%,84% and 76% respectively In group B,apoptotic cells stained with TUNEL method were found in delayed neuronal death after transient cerebral ischemia,but not found in group A Conclusion Ischemic preconditioning protects brain from subsequent ischemia insult Apoptosis may be enhanced by cerebral ischemia