新型聚乳酸/骨基质多孔复合支架材料制备及初步研究

目的采用CO2超临界法(SC-CO2)制备一种新型的聚乳酸(PLA)/骨基质(BMG)多孔复合型生物活性骨支架材料,并评价其理化性能及细胞相容性,确定材料最佳复合比例,为进一步的实验提供实验依据。方法采用SC-CO2法制备不同比例的PLA/BMG多孔复合型骨植入材料,通过大体观察、孔隙率测定,力学检测、SEM观察评价其理化性能,并结合体外细胞相容性检测选择最佳复合比例。结果采用SC-CO2法制备的PLA/BMG多孔复合支架材料中BMG的比例与材料的孔隙率及细胞相容性成正相关,与力学性能成负相关;含30%BMG的复合材料具有良好的综合性能。结论采用SC-CO2法制备的PLA/BMG(7/3)多孔复合支架材料具有良好的理化性能及细胞相容性,可作为骨植入材料及骨组织工程支架进一步研究。

Preparation of porous polylactic-acid/bone matrix gelatin composite as scaffold materials for bone-tissue engineering

Objective To develop a novel porous poly lactic-acid(PLA)/bone matrix gelatin(BMG)bioactive composite biomaterial with supercritical carbon dioxide(SC-CO2)extraction and determine the optimal proportion of the ingredients by evaluating the physical and chemical characteristics and cellular compatibility of the composites.Methods The PLA/BMG composite biomaterials with various PLA/BMG proportions were prepared with SC-CO2 technique and evaluated by macroscopic observation,porosity and mechanical assessment,and scanning electron micrograph(SEM).The optimal proportion of PLA/BMG was determined in conjunction with the results of cellular compatibility evaluation.Results BMG proportion in BLA/BMG composite biomaterials prepared with SC-CO2 technique was positively associated with the cellular compatibility and porosity,and inversely with the mechanics of the prepared composite.The results showed BMG content of 30% in PLA/BMG composite was optimal.Conclusion The composite with PLA and BMG proportion of 7:3 possesses good physical and chemical characteristics and cellular compatibility,suggesting its potential as the bone implant biomaterial and bone-tissue engineering scaffold.