卵巢癌患者化疗前后淋巴细胞亚群分析

目的 探讨化疗对卵巢癌患者免疫功能的影响.方法 收集卵巢癌行化疗患者34例(卵巢癌组),以同期体检的健康妇女30例作为对照组.检测两组外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD25+等指标,分析各淋巴细胞亚群情况.结果 卵巢癌组患者外周血CD3+、CD4+以及CD4+/CD8+均较对照组显著降低(0.5524±0.1121比0.6685±0.0869、0.3183±0.0864比0.4139±0.0524、1.06±0.29比1.43±0.21),CD4+CD25+较对照组显著上升(0.1026±0.0418比0.0287±0.0132),差异有统计学意义(P<0.05).卵巢癌组患者外周血淋巴细胞亚群化疗后1周与化疗前比较差异无统计学意义(P>0.05);化疗后3周CD3+、CD4+以及CD4+/CD8+均较化疗前显著升高,CD4+ CD25+较化疗前显著下降,差异有统计学意义(P<0.05).结论 卵巢癌患者体内淋巴细胞亚群比例失调,化疗可改善卵巢癌的免疫抑制状态,恢复正常的淋巴细胞亚群比例.

Abstract：

Objective To detect the effects of chemotherapy on the immune function of patients with ovarian cancer.Methods Thirty-four patients with ovarian cancer(ovarian cancer group)who accepted chemotherapy were collected.Thirty healthy women were used as control group.The lymphocyte sub-cluster CD3+,CD4+,CD8+ and CD25+of peripheral blood were detected by flow cytometry.Results The peripheral blood CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were lower than those in control group(0.5524±0.1121 vs.0.6685±0.0869,0.3183±0.0864 vs.0.4139±0.0524,1.06±0.29 vs.1.43±0.21),CD4+ CD25+was higher than that in control group(0.1026±0.0418 vs.0.0287±0.0132),there was significant difference between two groups(P<0.05).There was no significant changes observed 1 week after chemotherapy(P>0.05).Three weeks after chemotherapy,the CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were higher and CD4+ CD25+ was lower,there were significant difference between two groups(P<0.05).Conclusions The ratio of lymphocyte sub-cluster is imbalance in the patients with ovarian cancer.Chemotherapy can improve the immunosuppression condition and recovery the ratio of lymphocyte sub-cluster in the patients with ovarian cancer.

Analysis of lymphocyte sub-cluster in the patients with ovarian cancer around the chemotherapy

Objective To detect the effects of chemotherapy on the immune function of patients with ovarian cancer.Methods Thirty-four patients with ovarian cancer(ovarian cancer group)who accepted chemotherapy were collected.Thirty healthy women were used as control group.The lymphocyte sub-cluster CD3+,CD4+,CD8+ and CD25+of peripheral blood were detected by flow cytometry.Results The peripheral blood CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were lower than those in control group(0.5524±0.1121 vs.0.6685±0.0869,0.3183±0.0864 vs.0.4139±0.0524,1.06±0.29 vs.1.43±0.21),CD4+ CD25+was higher than that in control group(0.1026±0.0418 vs.0.0287±0.0132),there was significant difference between two groups(P<0.05).There was no significant changes observed 1 week after chemotherapy(P>0.05).Three weeks after chemotherapy,the CD3+,CD4+ and CD4+/CD8+ in ovarian cancer group were higher and CD4+ CD25+ was lower,there were significant difference between two groups(P<0.05).Conclusions The ratio of lymphocyte sub-cluster is imbalance in the patients with ovarian cancer.Chemotherapy can improve the immunosuppression condition and recovery the ratio of lymphocyte sub-cluster in the patients with ovarian cancer.