| Beagle犬血浆中尼莫地平的HPLC法测定及其药动学 |
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| 引用本文： | 宋海波,巩洪刚,杨书华,常建晖,邓树海.Beagle犬血浆中尼莫地平的HPLC法测定及其药动学[J].中国医药工业杂志,2009,40(10). |
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| 作者姓名： | 宋海波 巩洪刚 杨书华 常建晖 邓树海 |
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| 作者单位： | 宋海波(山东大学齐鲁医院临床药理研究所,山东济南,250012;山东省医药工业研究所);巩洪刚,杨书华,常建晖(济南宏瑞创博医药科技开发有限公司,山东济南,250100);邓树海(山东大学药学院药物制剂研究所,山东济南,250012) |
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| 摘 要： | 建立了HPLC法测定Beagle犬血浆中尼莫地平的含量,并研究了6只健康Beagle犬一日两次口服60 mg尼莫地平缓释片后的药动学.血浆样品经液-液萃取后测定,采用C18色谱柱,以乙腈-0.08mol/L乙酸铵(60:40)为流动相,检测波长358nm.尼莫地平在2.5～60μg/L浓度范围内线性关系良好,最低定量限为2.5μg/L,日内和日间 RSD均小于10%.主要药动学参数为:t1/2(5.6±0.7)h,c(39.0±3.6)μg/L,tmax(4.5±1.2)h,AUCo-t(626.0±34.0)μg·h·L-1,AUC0-∞(680.2±42.9)μg·h·L-1.
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| 关 键 词： | 尼莫地平 高效液相色谱 血药浓度 药动学 测定 |
Determination of Nimodipine in Beagle Dog Plasma by HPLC and Its Pharmacokinetics |
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| Abstract: | An HPLC method was established for the determination of nimodipine in Beagle dog plasma.The pharmacokinetics of six healthy Beagle dogs after oral administration of 60 mg (bid) nimodipine sustained-release tablets was investigated.The plasma samples were treated by liquid-liquid extraction.A C_(18) column was used,with the mobile phase of acetonitrile-0.08 mol/L ammonium acetate (60:40),at the detection wavelength of 358 nm.The calibration curve was linear in the concentration range of 2.5-60 μg/L.The low limit of quantifi cation was 2.5 μg/L.The RSDs of inter-and intra-day were lower than 10%.The main pharmacokinetic parameters were as follows:t_(1/2) (5.6±0.7) h,c_(max) (39.0±3.6) μg/L,t_(max) (4.5±1.2) h,AUC_(0-t) (626.0±34.0) μg·h·L~(-1),AUC_(0-∞) (680.2±42.9) μg·h·L~(-1). |
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| Keywords: | nimodipine HPLC plasma concentration pharmacokinetics determination |
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